Long-term exposure to moderate cold reduces incidence of reperfusion tachyarrhythmias in rats.

IF 3.3 3区 医学 Q1 PHYSIOLOGY
Pavel Vebr, Frantisek Galatík, Aneta Marvanova, Barbara Elsnicova, Daniela Hornikova, Marek Vecka, Sarka Spinova, Olga Novakova, Jitka M Zurmanova
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Abstract

Recently, moderate cold acclimation (9 °C; MCA) was found to exert cardioprotective effects by increasing resistance to ischemia-reperfusion (IR) injury and mitochondrial calcium overload in rats, but the effect of MCA on the incidence of IR arrhythmias is not known. The aim of this study was to determine whether MCA can induce an anti-arrhythmic effect and, if so, to elucidate a possible mechanism. Adult male Wistar rats were acclimated (9 °C) for short (1-3-10 days) and long-term (5 weeks; CA) periods, followed by a two-week recovery period (24 °C; CAR). The number of premature ventricular complexes (PVCs) and their duration after IR, western blotting, thin layer and gas chromatography were performed on left ventricular myocardium. We revealed that total reperfusion PVCs and tachyarrhythmia duration decreased even after CA, and accordingly the anti-arrhythmic n-3PUFAs increased in cardiac membrane phospholipids, the n-6/n-3 PUFA ratio decreased. CA increased the distribution of connexin 43 (Cx43) in favor of end-to-end junctions and the expression of uncoupling protein UCP3 in mitochondria. These beneficial effects were lost after two-weeks recovery period CAR. Interestingly, the mitochondrial antioxidants superoxide dismutase (SOD2) and thioredoxin reductase (TRXRD2) were strongly upregulated exclusively after 1 day of cold exposure, whereas cytosolic TRXRD1 was downregulated. In conclusion, long-term MCA (5-weeks) reduces the incidence of reperfusion arrhythmias, increases the proportion of anti-arrhythmic n-3PUFAs in cardiomyocyte membranes and has a positive effect on Cx43 distribution. By increasing the amount of UCP3 in mitochondria, it may reduce the likelihood of free radical formation in mitochondria during reperfusion.

长期暴露于中等低温环境可降低大鼠再灌注心律失常的发生率。
最近,中度冷驯化(9°C; MCA)被发现通过增加大鼠对缺血再灌注(IR)损伤的抵抗和线粒体钙超载来发挥心脏保护作用,但MCA对IR心律失常的发生率的影响尚不清楚。本研究的目的是确定MCA是否可以诱导抗心律失常的作用,如果是的话,阐明可能的机制。成年雄性Wistar大鼠进行短期(1-3-10天)和长期(5周;CA)适应期(9°C),然后进行为期两周的恢复期(24°C; CAR)。采用IR、western blotting、薄层和气相色谱法测定左心室早搏复合体(PVCs)的数量及持续时间。我们发现,即使在CA后,总再灌注室性早搏和快速心律失常持续时间也减少了,相应地心膜磷脂中抗心律失常的n- 3pufa增加,n-6/n-3 PUFA比值降低。CA增加了连接蛋白43 (Cx43)的分布,有利于端到端连接和线粒体中解偶联蛋白UCP3的表达。这些有益的效果在两周的恢复期CAR后就消失了。有趣的是,线粒体抗氧化剂超氧化物歧化酶(SOD2)和硫氧还蛋白还原酶(TRXRD2)在冷暴露1天后强烈上调,而细胞质TRXRD1则下调。综上所述,长期MCA(5周)可降低再灌注心律失常的发生率,增加心肌细胞膜抗心律失常n-3PUFAs的比例,并对Cx43的分布有积极影响。通过增加线粒体中UCP3的数量,可以降低再灌注时线粒体中自由基形成的可能性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
6.00
自引率
9.10%
发文量
296
审稿时长
2-4 weeks
期刊介绍: The Journal of Applied Physiology publishes the highest quality original research and reviews that examine novel adaptive and integrative physiological mechanisms in humans and animals that advance the field. The journal encourages the submission of manuscripts that examine the acute and adaptive responses of various organs, tissues, cells and/or molecular pathways to environmental, physiological and/or pathophysiological stressors. As an applied physiology journal, topics of interest are not limited to a particular organ system. The journal, therefore, considers a wide array of integrative and translational research topics examining the mechanisms involved in disease processes and mitigation strategies, as well as the promotion of health and well-being throughout the lifespan. Priority is given to manuscripts that provide mechanistic insight deemed to exert an impact on the field.
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