Sublethal exposure to benzalkonium chloride induced biocide-antibiotic cross-resistance in hypervirulent L. monocytogenes.

IF 3.2 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of Applied Microbiology Pub Date : 2025-10-06 DOI:10.1093/jambio/lxaf241

Joshua Macleod, Zak Hamid, Benjamin E Johns, Michael L Beeton, James Blaxland

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引用次数: 0

Abstract

Aims: Listeria monocytogenes is a foodborne pathogen found ubiquitously in the food manufacturing environment (FME), where exposure to biocides at sublethal concentrations may induce adaptation and cross-resistance. This study aimed to investigate whether exposure to sublethal benzalkonium chloride (BAC) concentrations induced cross-resistance to antibiotics in an adaptive laboratory evolution (ALE) model.

Methods and results: Six L. monocytogenes and two Listeria innocua strains underwent a 30-day sublethal BAC ALE. Susceptibility was performed against biocides and antibiotics. Variant calling was performed relative to wild-type strains. Three intragenic mutations and two intergenic mutations upstream of fepR, encoding a local repressor of the fluoroquinolone efflux pump FepA are identified in BAC-adapted isolates, associated with phenotypic reduced susceptibility to BAC, fluoroquinolones, and co-trimoxazole.

Conclusions: Sublethal BAC exposure can select for fepR-associated variants linked to reduced susceptibility to both biocides and antibiotics, highlighting concerns for biocide-antibiotic cross-resistance.

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亚致死暴露于苯扎氯铵诱导高毒性单核细胞增生乳杆菌的杀菌剂-抗生素交叉抗性。

目的：单核增生李斯特菌是一种在食品生产环境（FME）中普遍存在的食源性病原体，在食品生产环境中，暴露于亚致死浓度的杀菌剂可能诱导适应和交叉抗性。本研究旨在研究在适应性实验室进化模型中暴露于亚致死浓度的苯扎氯铵是否会诱导抗生素交叉耐药。方法和结果：6株单核增生乳杆菌和2株innocua乳杆菌进行了30天的亚致死BAC适应性实验室进化。对杀菌剂和抗生素进行药敏试验。相对于野生型菌株进行变异调用。在BAC适应的分离株中，鉴定出fepR上游的3个基因内突变和2个基因间突变，编码氟喹诺酮类外排泵FepA的局部抑制因子，与表型上对BAC、氟喹诺酮类药物和复方新诺明的敏感性降低有关。结论：亚致死BAC暴露可以选择与杀菌剂和抗生素敏感性降低相关的fepr相关变异，突出了对杀菌剂-抗生素交叉耐药的关注。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Applied Microbiology 生物-生物工程与应用微生物

CiteScore

7.30

自引率

2.50%

发文量

427

审稿时长

2.7 months

期刊介绍： Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.