{"title":"Iron-Oxide Labeled Stem Cells with Specific Magnetic Occurrence for Effective in Mouse Model of Cisplatin-Induced Acute Kidney Injury.","authors":"Ke Wang, Ye Zhao, Huiying Lv, Xiuying Li","doi":"10.2147/IJN.S528752","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Acute kidney injury (AKI) is characterized by the abrupt loss of renal function and lack of curative therapies. Placental-derived mesenchymal stem cells (PL-MSCs) have shown promise in regenerative medicine, including in the treatment of AKI. However, optimizing the therapeutic effects of PL-MSCs remains a critical objective. Magnetic targeting is one potential avenue of optimization. Using iron oxide-labeled MSCs with an external magnetic field to increase cell homing ability may be an ideal method for improving the cell therapy effects in vivo.</p><p><strong>Methods: </strong>In this study, PL-MSCs were labeled with Fe<sub>3</sub>O<sub>4</sub> nanoparticles coated with polydopamine (Fe<sub>3</sub>O<sub>4</sub>@PDA NPs) for 24 h, and cell efficiency and viability were tested. The conditionally immortalized mice renal tubular endothelial cells (mRTECs) were incubated with cisplatin (Cis) and co-cultured with non-labeled or NP-labeled MSCs. The protective effect of NP-labeled MSCs on mRTEC was evaluated. In in vivo experiments, non-labeled or NP-labeled MSCs, with or without an external magnetic field, were injected into mice with Cis-induced AKI. The blood and tissue samples were collected to assess renal function and tissue damage.</p><p><strong>Results: </strong>The study confirmed that MSCs or MSC-NP can significantly improve Cis-induced mRTEC injury. In addition, NP-labeled MSCs with an external magnetic field (magnetically-targeted MSCs) improved their homing to the kidney tissues in mice with AKI, resulting in enhanced kidney function compared with those of mice treated with MSC or NP-labeled MSC treatment alone. Moreover, magnetically-targeted MSCs alleviated renal injury through suppressing oxidative stress and inflammation, reducing cell apoptosis, and promoting cell proliferation.</p><p><strong>Conclusion: </strong>Magnetic targeting enhances the therapeutic effects of PL-MSCs on Cis-induced AKI in mice, suggesting that magnetically-targeted MSCs could serve as potential treatments for patients with Cis-induced AKI.</p>","PeriodicalId":14084,"journal":{"name":"International Journal of Nanomedicine","volume":"20 ","pages":"11435-11449"},"PeriodicalIF":6.5000,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12452962/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Nanomedicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2147/IJN.S528752","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"NANOSCIENCE & NANOTECHNOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Acute kidney injury (AKI) is characterized by the abrupt loss of renal function and lack of curative therapies. Placental-derived mesenchymal stem cells (PL-MSCs) have shown promise in regenerative medicine, including in the treatment of AKI. However, optimizing the therapeutic effects of PL-MSCs remains a critical objective. Magnetic targeting is one potential avenue of optimization. Using iron oxide-labeled MSCs with an external magnetic field to increase cell homing ability may be an ideal method for improving the cell therapy effects in vivo.
Methods: In this study, PL-MSCs were labeled with Fe3O4 nanoparticles coated with polydopamine (Fe3O4@PDA NPs) for 24 h, and cell efficiency and viability were tested. The conditionally immortalized mice renal tubular endothelial cells (mRTECs) were incubated with cisplatin (Cis) and co-cultured with non-labeled or NP-labeled MSCs. The protective effect of NP-labeled MSCs on mRTEC was evaluated. In in vivo experiments, non-labeled or NP-labeled MSCs, with or without an external magnetic field, were injected into mice with Cis-induced AKI. The blood and tissue samples were collected to assess renal function and tissue damage.
Results: The study confirmed that MSCs or MSC-NP can significantly improve Cis-induced mRTEC injury. In addition, NP-labeled MSCs with an external magnetic field (magnetically-targeted MSCs) improved their homing to the kidney tissues in mice with AKI, resulting in enhanced kidney function compared with those of mice treated with MSC or NP-labeled MSC treatment alone. Moreover, magnetically-targeted MSCs alleviated renal injury through suppressing oxidative stress and inflammation, reducing cell apoptosis, and promoting cell proliferation.
Conclusion: Magnetic targeting enhances the therapeutic effects of PL-MSCs on Cis-induced AKI in mice, suggesting that magnetically-targeted MSCs could serve as potential treatments for patients with Cis-induced AKI.
期刊介绍：
The International Journal of Nanomedicine is a globally recognized journal that focuses on the applications of nanotechnology in the biomedical field. It is a peer-reviewed and open-access publication that covers diverse aspects of this rapidly evolving research area.
With its strong emphasis on the clinical potential of nanoparticles in disease diagnostics, prevention, and treatment, the journal aims to showcase cutting-edge research and development in the field.
Starting from now, the International Journal of Nanomedicine will not accept meta-analyses for publication.
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