Assessment of a rapid diagnostic test based on loop-mediated isothermal amplification (LAMP) to identify the most frequent pathogens causing hospital-acquired pneumonia.
Anna Sellarès-Crous, Arturo Martínez-Trejo, Natàlia Arnalda-Muñoz, Giulia Gatti, Miriam Villanueva-López, Andrea Vergara-Gómez, Francesc Marco-Reverté, Mateu Espasa-Soley, Jordi Vila-Estapé
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引用次数: 0
Abstract
Introduction: Hospital-acquired pneumonia (HAP) is a serious infection affecting patients in the hospital setting. This study aimed to evaluate a novel multiplex detection method using loop-mediated isothermal amplification (LAMP) technology to identify six primary bacterial pathogens responsible for HAP directly from respiratory samples.
Methods: A total of 119 clinical samples were analyzed by LAMP technology, including mainly bronchoalveolar lavages, endotracheal aspirates, and bronchoaspirates.
Results and discussion: The results of the LAMP and traditional culture methods showed an accuracy of 93.0%. In some discordant cases between culture and LAMP, multiplex PCR (FilmArray Pneumonia Panel) showed a strong correlation with the LAMP results, confirming the potential use of this technique as a diagnostic detection tool. The clinical sensitivity of the LAMP assay was 93.3% with a specificity of 92.0%. Correlation analysis revealed a weak negative relationship between bacterial load and time to positivity (r = -0.177, p = 0.05). This study underscores the potential of LAMP as a rapid and accurate tool for the diagnosis of HAP, facilitating the turnaround time for microbiology laboratory results, which is critical for improving the outcomes of patients with HAP.
期刊介绍：
Frontiers in Cellular and Infection Microbiology is a leading specialty journal, publishing rigorously peer-reviewed research across all pathogenic microorganisms and their interaction with their hosts. Chief Editor Yousef Abu Kwaik, University of Louisville is supported by an outstanding Editorial Board of international experts. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.
Frontiers in Cellular and Infection Microbiology includes research on bacteria, fungi, parasites, viruses, endosymbionts, prions and all microbial pathogens as well as the microbiota and its effect on health and disease in various hosts. The research approaches include molecular microbiology, cellular microbiology, gene regulation, proteomics, signal transduction, pathogenic evolution, genomics, structural biology, and virulence factors as well as model hosts. Areas of research to counteract infectious agents by the host include the host innate and adaptive immune responses as well as metabolic restrictions to various pathogenic microorganisms, vaccine design and development against various pathogenic microorganisms, and the mechanisms of antibiotic resistance and its countermeasures.
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