Identification and antimicrobial resistance profile of Enterobacterales and Staphylococcus spp. isolated from subclinical mastitis milk.

IF 1.1 4区综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES

Anais da Academia Brasileira de Ciencias Pub Date : 2025-09-19 eCollection Date: 2025-01-01 DOI:10.1590/0001-3765202520241287

Juliana S Alves, Rossiane M Souza, Jeniffer F Miranda, Guilherme C L DA Silva, Jéssica P L Moreira, Alice G M Gonzalez

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Abstract

This study aimed to identify and evaluate the antimicrobial resistance profile of Enterobacterales and Staphylococcus spp. isolated from raw milk samples from mammary quarters with subclinical mastitis (SCM), from three small dairy farms suppliers of raw milk for cheese production, in Rio de Janeiro state. Raw milk from each mammary quarter was investigated for signs of SCM using the California Mastitis Test (CMT). Raw milk with signs of SCM (SCMM) was seeded on Compact-Dry EC and Compact Dry X-SA. The strains were identified by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) and evaluated for antimicrobial resistance, by disk diffusion test. SCM was observed in 13 (8.02%) mammary quarters from farm A, 24 (25%) from farm B and 7 (7.95%) from farm C. Staphylococcus aureus was the most isolated on farms A (55.56%) and B (75.00%). Enterobacter roggenkampii was isolated for the first time from SCMM, being the most prevalent specie on farm C (50.00%). Methicillin-resistant Staphylococcus aureus (MRSA) and Methicillin-resistant Staphylococcus chromogenes (MRS) were isolated from SCMM from farms A and B. The health of the dairy herd requires great attention, mainly reflected in the control of MRSA/MRS.

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亚临床乳腺炎乳分离肠杆菌和葡萄球菌的鉴定及耐药性分析。

本研究旨在鉴定和评估巴西巴西州三个小型奶牛场的亚临床乳腺炎（SCM）乳区原料奶样本中分离的肠杆菌和葡萄球菌的抗微生物药物耐药性。这些奶区为奶酪生产提供原料奶。使用加州乳腺炎试验（CMT）对来自每个乳腺区域的生乳进行检查，以寻找SCM的迹象。在Compact-Dry EC和Compact Dry X-SA上播种有SCM （SCMM）迹象的原料奶。采用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）对菌株进行鉴定，并采用纸片扩散试验对菌株进行耐药性评价。A场13间（8.02%）、B场24间（25%）、c场7间（7.95%）分别检出金黄色葡萄球菌，其中A场（55.56%）和B场（75.00%）检出最多。罗根坎皮肠杆菌首次从SCMM中分离到，是C农场最常见的菌种（50.00%）。从A场和b场SCMM中分离到耐甲氧西林金黄色葡萄球菌（MRSA）和耐甲氧西林显色葡萄球菌（MRS），需要高度关注奶牛群的健康状况，主要体现在MRSA/MRS的控制上。

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来源期刊

Anais da Academia Brasileira de Ciencias 综合性期刊-综合性期刊

CiteScore

2.20

自引率

0.00%

发文量

347

审稿时长

1 months

期刊介绍： The Brazilian Academy of Sciences (BAS) publishes its journal, Annals of the Brazilian Academy of Sciences (AABC, in its Brazilianportuguese acronym ), every 3 months, being the oldest journal in Brazil with conkinuous distribukion, daking back to 1929. This scienkihic journal aims to publish the advances in scienkihic research from both Brazilian and foreigner scienkists, who work in the main research centers in the whole world, always looking for excellence. Essenkially a mulkidisciplinary journal, the AABC cover, with both reviews and original researches, the diverse areas represented in the Academy, such as Biology, Physics, Biomedical Sciences, Chemistry, Agrarian Sciences, Engineering, Mathemakics, Social, Health and Earth Sciences.