Rhabdovirus Glycoprotein Triggers Janus Kinase/Signal Transducer and Activator of Transcription Antiviral Immunity via Direct Recognition by Insect Dome Receptor.

IF 6.3 1区 生物学 Q1 PLANT SCIENCES
Tingting Yu, You Li, Mengjia Xu, Zhihao Wan, Dongsheng Jia, Taiyun Wei
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Abstract

Janus kinase/signal transducer and activator of transcription (JAK/STAT) immune response traditionally requires cytokine binding to receptors for activation; however, it remains unclear whether cytokine receptors function as direct pattern recognition receptors (PRRs) that recognise viral envelopes. In this study, we demonstrate that cytokine receptor Domeless (Dome) in leafhopper Recilia dorsalis directly recognises glycoprotein (G) of an important rice rhabdovirus. This binding induces Dome dimerisation, which initiates JAK/STAT pathway and leads to STAT-dependent expression of antiviral immune effector in R. dorsalis (RdIE1). RdIE1 suppresses viral replication by inhibiting the RNA-binding activity of viral large polymerase (L). Dome residue T659 is essential for G recognition and L residues E774 and D775 are required for RdIE1 target. Notably, virus-encoded phosphoprotein competitively inhibits the phosphorylation of STAT by casein kinase II (CK2β), thereby abrogating STAT-dependent transactivation of target genes. Our findings redefine Dome as a viral sensor and reveal a biphasic strategy that balances antiviral immunity with persistent viral infection in insect vectors.

横纹肌病毒糖蛋白通过昆虫圆顶受体直接识别触发Janus激酶/信号转导和转录激活因子的抗病毒免疫。
Janus激酶/信号转导和转录激活因子(JAK/STAT)免疫反应传统上需要细胞因子结合受体激活;然而,目前尚不清楚细胞因子受体是否作为识别病毒包膜的直接模式识别受体(PRRs)起作用。在这项研究中,我们证明了叶蝉的细胞因子受体Domeless (Dome)直接识别一种重要的水稻横纹病病毒的糖蛋白(G)。这种结合诱导了Dome二聚化,启动了JAK/STAT通路,并导致了dorsalis中抗病毒免疫效应物的STAT依赖表达(RdIE1)。RdIE1通过抑制病毒大聚合酶(L)的rna结合活性来抑制病毒复制。圆顶残基T659是G识别所必需的,L残基E774和D775是RdIE1目标所必需的。值得注意的是,病毒编码的磷酸化蛋白竞争性地抑制酪蛋白激酶II (CK2β)对STAT的磷酸化,从而消除了STAT依赖的靶基因的转激活。我们的发现重新定义了Dome作为一种病毒传感器,并揭示了一种平衡抗病毒免疫与昆虫载体持续病毒感染的双相策略。
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来源期刊
Plant, Cell & Environment
Plant, Cell & Environment 生物-植物科学
CiteScore
13.30
自引率
4.10%
发文量
253
审稿时长
1.8 months
期刊介绍: Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.
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