Sesame oil ameliorates aspartame-induced immunotoxicity in mice: a hematological, antioxidative, and histopathological evaluation

IF 2.2 4区生物学 Q3 CELL BIOLOGY

Journal of Molecular Histology Pub Date : 2025-09-24 DOI:10.1007/s10735-025-10612-2

Khola Basalat, Ata Ul Mustafa Fahad, Amber Zahid, Maryam Iqbal, Sara Tariq, Laraib Zahid

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Abstract

Aspartame is a widely used non-nutritive sweetener suspected of having immunotoxic effects. This study evaluated the protective role of sesame oil against aspartame-induced toxicity in Swiss albino mice. Male Swiss albino mice were divided into four groups (n = 40): control, aspartame-treated (40 mg/kg/day), aspartame + sesame oil, and sesame oil alone. Over 60 days, aspartame (ASP) administration resulted in increased body weight, feed, and water intake, alongside a reduction in relative organ weights. Hematological analysis showed a significant decline in WBCs, eosinophils, and monocytes in the ASP group. Antioxidant activity (GSH, GPx) was significantly impaired in ASP-treated mice. Histopathological analysis revealed structural anomalies in both thymus and spleen, including capsular thickening, trabecular enlargement, white pulp degeneration, and increased apoptotic macrophages in the thymic cortex. Co-administration of sesame oil ameliorated these toxic effects, with notable improvements in organ morphology, hematological parameters, and histological integrity. Mice treated with sesame oil alone showed no significant deviations from control values. These findings suggest that sesame oil may offer protective effects against aspartame-induced immunotoxicity, possibly via its antioxidant properties.

Graphical abstract

Methodology of the experiment

Abstract Image

查看原文本刊更多论文

香油改善小鼠阿斯巴甜诱导的免疫毒性：血液学、抗氧化和组织病理学评估。

阿斯巴甜是一种广泛使用的非营养性甜味剂，被怀疑具有免疫毒性作用。本研究评价了香油对阿斯巴甜引起的瑞士白化小鼠毒性的保护作用。将雄性瑞士白化小鼠分为4组（n = 40）：对照组、阿斯巴甜处理组（40 mg/kg/天）、阿斯巴甜+香油组和单独使用香油组。超过60天，阿斯巴甜（ASP）的施用导致体重增加，饲料和水的摄入量增加，同时相对器官重量减少。血液学分析显示，ASP组白细胞、嗜酸性粒细胞和单核细胞显著下降。抗氧化活性（GSH, GPx）显著降低。组织病理学分析显示胸腺和脾脏结构异常，包括囊增厚，小梁增大，白色髓变性，胸腺皮质凋亡巨噬细胞增加。同时使用香油可以改善这些毒性作用，并显著改善器官形态、血液学参数和组织完整性。单独用香油处理的小鼠与控制值没有明显的偏差。这些发现表明，芝麻油可能对阿斯巴甜引起的免疫毒性具有保护作用，可能是通过其抗氧化特性。

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来源期刊

Journal of Molecular Histology 生物-细胞生物学

CiteScore

5.90

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Molecular Histology publishes results of original research on the localization and expression of molecules in animal cells, tissues and organs. Coverage includes studies describing novel cellular or ultrastructural distributions of molecules which provide insight into biochemical or physiological function, development, histologic structure and disease processes. Major research themes of particular interest include: - Cell-Cell and Cell-Matrix Interactions; - Connective Tissues; - Development and Disease; - Neuroscience. Please note that the Journal of Molecular Histology does not consider manuscripts dealing with the application of immunological or other probes on non-standard laboratory animal models unless the results are clearly of significant and general biological importance. The Journal of Molecular Histology publishes full-length original research papers, review articles, short communications and letters to the editors. All manuscripts are typically reviewed by two independent referees. The Journal of Molecular Histology is a continuation of The Histochemical Journal.