Nanoparticle-loaded dsRNA delivery system for pesticides: status and perspective

Abstract

The integration of RNA interference (RNAi) technology with nanotechnology shows significant potential in overcoming the limitations of traditional double-stranded RNA (dsRNA) delivery systems, thereby enabling more efficient dsRNA delivery. Nanoparticles for dsRNA delivery have the potential to enhance the efficiency of RNAi and improve system stability. In this study, we discuss the limitations of conventional RNAi-based biopesticides, systematically introduce common nanoparticle carriers used for RNA pesticide delivery, analyze the interactions between nanoparticles and dsRNA during the delivery, and finally emphasize the overall limitations, associated risks, and challenges in practical applications.