Ana Santos-Coquillat, Beatriz G. Neves, Raquel C. Gonçalves, Dora C.S. Costa, João F. Mano, Mariana B. Oliveira
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引用次数: 0
Abstract
Proteins serve as bioactive components and primary building blocks of hydrogels for bioengineering. While extracellular matrices and blood derivatives from animals and humans have been widely explored, the use of in vitro-cultured cells as bioactive sources remains limited. The cell secretome holds therapeutic potential administered in its liquid form and has been previously incorporated within exogenous materials that confer structural features to secretome-enriched devices. Producing hydrogels exclusively from cell-secreted sources could enhance the predictability, potency, and autologous character of these technologies. Here, we suggest the processing of secretomes derived from adipose-tissue-derived mesenchymal stromal cells (MSCs) and their further crosslinking into volumetric structures. Secretomes from pristine and interferon-γ (IFN-γ)-primed MSCs showed different protein compositions, with high reproducibility between production cycles, generalized high cytocompatibility, and pro-angiogenic activity. The platform introduced herein may be used to explore secretomes of different cell types, targeting applications that may benefit from their therapeutic and biotechnological potential.
期刊介绍:
Matter, a monthly journal affiliated with Cell, spans the broad field of materials science from nano to macro levels,covering fundamentals to applications. Embracing groundbreaking technologies,it includes full-length research articles,reviews, perspectives,previews, opinions, personnel stories, and general editorial content.
Matter aims to be the primary resource for researchers in academia and industry, inspiring the next generation of materials scientists.