Time-Resolved Small-Angle X-ray Scattering Studies of pH-Induced PMPC-PDPA Diblock Copolymer Self-Assembly.

IF 3.9 2区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Langmuir Pub Date : 2025-09-24 DOI:10.1021/acs.langmuir.5c03950

Guoxing Liao,Daniel T W Toolan,Nicholas J Warren,Paul D Topham,Oleksandr O Mykhaylyk,Yunjie Zhang,Nicholas J Terrill,LinGe Wang

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Abstract

Despite the potential of poly(2-methacryloyloxy ethyl phosphorylcholine)-poly(2-(diisopropylamino)ethyl methacrylate) (PMPC-PDPA) diblock copolymer nanoparticles for several biological applications, the exact mechanism of pH-induced self-assembly of the PMPC-PDPA chains into nanoparticles remains unclear, although it has been extensively studied by ex situ transmission electron microscopy. Here, we probe this process using time-resolved small-angle X-ray scattering (TR-SAXS) to gain an understanding of the phenomena that occur on the nanoscale. Modeling the TR-SAXS data indicated that spherical micelles and vesicles were formed at a pH as low as 3, and the spherical micelle and vesicle structures reformed at pH 5.5. At pH ∼5.5, insoluble PMPC25-PDPA70 diblock copolymer precipitation was also observed by SAXS. A huge soluble PMPC25-PDPA70 diblock copolymer reservoir might assist in PMPC25-PDPA70 vesicle construction. Additionally, a potential pathway of vesicle construction by spherical micelle fusion was supported by the SAXS evidence.

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ph诱导PMPC-PDPA双嵌段共聚物自组装的时间分辨小角x射线散射研究。

尽管聚（2-甲基丙烯酰氧基乙基磷胆碱）-聚（2-（二异丙胺）乙基甲基丙烯酸酯）（PMPC-PDPA）二嵌段共聚物纳米颗粒在几种生物学应用中具有潜力，但ph诱导PMPC-PDPA链自组装成纳米颗粒的确切机制尚不清楚，尽管它已经通过非原位透射电子显微镜进行了广泛的研究。在这里，我们使用时间分辨小角度x射线散射（TR-SAXS）来探测这一过程，以了解发生在纳米尺度上的现象。对r - saxs数据的模拟表明，pH低至3时，球形胶束和囊泡形成，pH为5.5时，球形胶束和囊泡结构发生改变。在pH ~ 5.5下，SAXS也观察到不溶性PMPC25-PDPA70二嵌段共聚物的沉淀。一个巨大的可溶PMPC25-PDPA70二嵌段共聚物储层可能有助于PMPC25-PDPA70囊泡的构建。此外，SAXS证据支持球形胶束融合构建囊泡的潜在途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Langmuir 化学-材料科学：综合

CiteScore

6.50

自引率

10.30%

发文量

1464

审稿时长

2.1 months

期刊介绍： Langmuir is an interdisciplinary journal publishing articles in the following subject categories: Colloids: surfactants and self-assembly, dispersions, emulsions, foams Interfaces: adsorption, reactions, films, forces Biological Interfaces: biocolloids, biomolecular and biomimetic materials Materials: nano- and mesostructured materials, polymers, gels, liquid crystals Electrochemistry: interfacial charge transfer, charge transport, electrocatalysis, electrokinetic phenomena, bioelectrochemistry Devices and Applications: sensors, fluidics, patterning, catalysis, photonic crystals However, when high-impact, original work is submitted that does not fit within the above categories, decisions to accept or decline such papers will be based on one criteria: What Would Irving Do? Langmuir ranks #2 in citations out of 136 journals in the category of Physical Chemistry with 113,157 total citations. The journal received an Impact Factor of 4.384*. This journal is also indexed in the categories of Materials Science (ranked #1) and Multidisciplinary Chemistry (ranked #5).