{"title":"Visualizing and mapping cell death: Biochemical tools, techniques and assays.","authors":"Sadhna Soni, Ashwini Nath Tiwari, Amrendra Singh, Arun Upadhyay","doi":"10.1016/bs.pmbts.2025.06.018","DOIUrl":null,"url":null,"abstract":"<p><p>Cell death is a fundamental process that plays a role in the development of multicellular organisms, tissue homeostasis, and fighting infections. Dysfunctional cell death signaling is associated with many diseases, including cancer. Most studied among multiple possible cell death pathways is apoptosis that is essential for various biological functions, including embryogenesis, aging, and the development of numerous diseases. This regulated cell death enables the removal of cells in a controlled manner, maintaining tissue homeostasis and aiding organismal development. Understanding cell death pathways can help develop new therapeutic strategies for treating diseases like cancer. For example, tumor cells often avoid apoptosis, which can lead to treatment resistance. Various groups of researchers believe that studying other cell death pathways, like necroptosis, pyroptosis, ferroptosis, and cuproptosis, may have high potential for cancer therapy. Numerous biochemical methods exist to detect, quantify, and analyze cell death pathways, each with unique principles, advantages, and limitations. A comprehensive understanding of these methods enables researchers to select appropriate techniques for their experimental contexts. This chapter systematically discusses the molecular and cellular changes related to various cell death pathways and the conventional and non-conventional methods used to investigate these processes.</p>","PeriodicalId":49280,"journal":{"name":"Progress in Molecular Biology and Translational Science","volume":"217 ","pages":"81-107"},"PeriodicalIF":0.0000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Progress in Molecular Biology and Translational Science","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1016/bs.pmbts.2025.06.018","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/7/5 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0
Abstract
Cell death is a fundamental process that plays a role in the development of multicellular organisms, tissue homeostasis, and fighting infections. Dysfunctional cell death signaling is associated with many diseases, including cancer. Most studied among multiple possible cell death pathways is apoptosis that is essential for various biological functions, including embryogenesis, aging, and the development of numerous diseases. This regulated cell death enables the removal of cells in a controlled manner, maintaining tissue homeostasis and aiding organismal development. Understanding cell death pathways can help develop new therapeutic strategies for treating diseases like cancer. For example, tumor cells often avoid apoptosis, which can lead to treatment resistance. Various groups of researchers believe that studying other cell death pathways, like necroptosis, pyroptosis, ferroptosis, and cuproptosis, may have high potential for cancer therapy. Numerous biochemical methods exist to detect, quantify, and analyze cell death pathways, each with unique principles, advantages, and limitations. A comprehensive understanding of these methods enables researchers to select appropriate techniques for their experimental contexts. This chapter systematically discusses the molecular and cellular changes related to various cell death pathways and the conventional and non-conventional methods used to investigate these processes.
期刊介绍:
Progress in Molecular Biology and Translational Science (PMBTS) provides in-depth reviews on topics of exceptional scientific importance. If today you read an Article or Letter in Nature or a Research Article or Report in Science reporting findings of exceptional importance, you likely will find comprehensive coverage of that research area in a future PMBTS volume.