Visualizing and mapping cell death: Biochemical tools, techniques and assays.

3区 生物学 Q2 Biochemistry, Genetics and Molecular Biology
Sadhna Soni, Ashwini Nath Tiwari, Amrendra Singh, Arun Upadhyay
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引用次数: 0

Abstract

Cell death is a fundamental process that plays a role in the development of multicellular organisms, tissue homeostasis, and fighting infections. Dysfunctional cell death signaling is associated with many diseases, including cancer. Most studied among multiple possible cell death pathways is apoptosis that is essential for various biological functions, including embryogenesis, aging, and the development of numerous diseases. This regulated cell death enables the removal of cells in a controlled manner, maintaining tissue homeostasis and aiding organismal development. Understanding cell death pathways can help develop new therapeutic strategies for treating diseases like cancer. For example, tumor cells often avoid apoptosis, which can lead to treatment resistance. Various groups of researchers believe that studying other cell death pathways, like necroptosis, pyroptosis, ferroptosis, and cuproptosis, may have high potential for cancer therapy. Numerous biochemical methods exist to detect, quantify, and analyze cell death pathways, each with unique principles, advantages, and limitations. A comprehensive understanding of these methods enables researchers to select appropriate techniques for their experimental contexts. This chapter systematically discusses the molecular and cellular changes related to various cell death pathways and the conventional and non-conventional methods used to investigate these processes.

可视化和绘制细胞死亡:生化工具、技术和分析。
细胞死亡是多细胞生物发育、组织稳态和抵抗感染的基本过程。功能失调的细胞死亡信号与包括癌症在内的许多疾病有关。在多种可能的细胞死亡途径中,研究最多的是细胞凋亡,它对多种生物功能至关重要,包括胚胎发生、衰老和许多疾病的发生。这种受调节的细胞死亡使细胞以受控的方式移除,维持组织稳态并帮助生物体发育。了解细胞死亡途径可以帮助开发治疗癌症等疾病的新治疗策略。例如,肿瘤细胞往往避免凋亡,这可能导致治疗耐药性。不同的研究小组认为,研究其他细胞死亡途径,如坏死性死亡、焦亡、铁亡和铜亡,可能具有很高的癌症治疗潜力。存在许多生物化学方法来检测、量化和分析细胞死亡途径,每种方法都有其独特的原理、优势和局限性。对这些方法的全面了解使研究人员能够为他们的实验环境选择适当的技术。本章系统地讨论了与各种细胞死亡途径相关的分子和细胞变化,以及用于研究这些过程的常规和非常规方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
5.00
自引率
0.00%
发文量
110
审稿时长
4-8 weeks
期刊介绍: Progress in Molecular Biology and Translational Science (PMBTS) provides in-depth reviews on topics of exceptional scientific importance. If today you read an Article or Letter in Nature or a Research Article or Report in Science reporting findings of exceptional importance, you likely will find comprehensive coverage of that research area in a future PMBTS volume.
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