Neural network reconstruction and motor function recovery in traumatic brain injury rat models via a 3D collagen/heparan/bFGF/NGF scaffold combined with mesenchymal stem cells COMBINED SCAFFOLD-MSC THERAPY FOR TBI REPAIR.

IF 3.8
Miao Chen, Yichao Ye, Tiezhu Wang, Xin Zhang, Jian Chen, Jian Zhang
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Abstract

Objective.To address the limited innate regenerative capacity of neural tissues following traumatic brain injury (TBI) by developing a novel therapeutic intervention.Approach.We engineered a composite scaffold using 3D bioprinting to integrate mesenchymal stem cells (MSCs) with collagen-heparan matrices supplemented with basic fibroblast growth factor (bFGF) and nerve growth factor (NGF), creating a 3D-CH-bFGF/NGF-MSCs construct.Main results.The engineered construct demonstrated favorable biomechanical characteristics and cytocompatibility. In rat TBI models, this intervention significantly enhanced cognitive recovery and sustained sensorimotor function restoration. Histopathological analyses revealed corresponding neural network regeneration through axonal regrowth, synaptogenesis reinforcement, and myelination enhancement at injury sites.Significance. This study demonstrates the therapeutic potential of a 3D-bioprinted, growth factor-enhanced MSC-scaffold construct to promote structural and functional neural repair after TBI, offering a promising strategy for neural tissue regeneration.

胶原/肝素/bFGF/NGF复合间充质干细胞对创伤性脑损伤大鼠模型神经网络重建和运动功能恢复的影响
目的:通过开发一种新的治疗干预方法来解决创伤性脑损伤(TBI)后神经组织先天再生能力有限的问题。方法:我们利用3D生物打印技术设计了一种复合支架,将间充质干细胞(MSCs)与补充碱性成纤维细胞生长因子(bFGF)和神经生长因子(NGF)的胶原-肝素基质结合起来,构建了3D- ch -bFGF/NGF-MSCs结构。主要结果:工程构建体表现出良好的生物力学特性和细胞相容性。在大鼠TBI模型中,这种干预显著增强了认知恢复和持续的感觉运动功能恢复。组织病理学分析显示相应的神经网络再生通过轴突再生、突触发生增强和损伤部位髓鞘形成增强。意义:本研究展示了3d生物打印、生长因子增强的MSC-scaffold构建促进TBI后神经结构和功能修复的治疗潜力,为神经组织再生提供了一种有前景的策略。
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