Metabolite-enhanced normothermic machine perfusion improves kidney transplant viability.

IF 6.1 1区医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL

JCI insight Pub Date : 2025-09-23 DOI:10.1172/jci.insight.190185

Jan Czogalla, Fabian Hausmann, Simon Lagies, Sydney E Gies, Sabrina Christiansen, Nico Kaiser, Fabian Haas, Yusuke Okabayashi, Dominik Kylies, Smilla Hofmann, Rossana Franzin, Niklas Sabra, Sarah Bouari, Yitian Fang, Gisela Ambagtsheer, Ilka Edenhofer, Silvia Chilla, Anne K Mühlig, Marina Zimmermann, Milagros N Wong, Takashi Yokoo, Oliver Kretz, Maja Lindenmeyer, Florian Grahammer, Martin J Hoogduijn, Ron de Bruin, Malte Kuehl, Sonja Hänzelmann, Bernd Kammerer, Loreto Gesualdo, Stefan Bonn, Robert C Minnee, Tobias B Huber, Victor G Puelles

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引用次数: 0

Abstract

Normothermic machine perfusion (NMP) has become a valuable tool to expand the pool of transplantable organs. However, the application of NMP to kidneys presents substantial challenges, mostly due to high variability in the composition of currently used perfusion solutions. Here, we provide a multimodal cross-species cellular atlas of kidney injury associated with NMP using a literature-based consensus buffer. This resource provided a systematic framework that was used to develop a metabolite-enhanced perfusion solution, which protected renal proximal tubular cells, improving cellular viability and transplantation outcomes across species, including human kidneys.

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代谢物增强的恒温机灌注改善肾移植生存能力。

常温机器灌注（NMP）已成为扩大可移植器官池的一种有价值的工具。然而，NMP在肾脏中的应用面临着巨大的挑战，主要是由于目前使用的灌注溶液组成的高度可变性。在这里，我们使用基于文献的共识缓冲提供了与NMP相关的肾损伤的多模态跨物种细胞图谱。该资源提供了一个系统框架，用于开发代谢物增强灌注溶液，该溶液可以保护肾近端小管细胞，提高细胞活力和跨物种移植结果，包括人类肾脏。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

JCI insight Medicine-General Medicine

CiteScore

13.70

自引率

1.20%

发文量

543

审稿时长

6 weeks

期刊介绍： JCI Insight is a Gold Open Access journal with a 2022 Impact Factor of 8.0. It publishes high-quality studies in various biomedical specialties, such as autoimmunity, gastroenterology, immunology, metabolism, nephrology, neuroscience, oncology, pulmonology, and vascular biology. The journal focuses on clinically relevant basic and translational research that contributes to the understanding of disease biology and treatment. JCI Insight is self-published by the American Society for Clinical Investigation (ASCI), a nonprofit honor organization of physician-scientists founded in 1908, and it helps fulfill the ASCI's mission to advance medical science through the publication of clinically relevant research reports.