Analytical capability of Raman spectroscopy to detect biochemical changes in red blood cell membrane disorders

IF 4.3 3区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Methods Pub Date : 2025-09-20 DOI:10.1016/j.ymeth.2025.09.007

Panchanil Sarmah , Ruchee Khanna , Aseefhali Bankapur

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引用次数: 0

Abstract

Detection of RBC membrane disorders with currently available modalities, such as osmotic fragility test (OFT), EMA binding test, ektacytometry, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), and next-generation sequencing (NGS), has been challenging as they either lack biochemical inferences or are complex in nature. Raman spectroscopy, a highly analytical method known to produce molecular fingerprints, has proven potential in extracting biochemical information from single individual cells. Recent advancements in membrane-targeted Raman measurements using excitation spots of donut and line intensity profiles can transform lab-on-chip Raman-activated cell sorting methods into a potential technique for RBC membrane disorder diagnosis. We, therefore, conjecture that Raman spectroscopy can be a strong contender as a diagnostic modality in RBC membranopathies. In this comprehensive review, we have attempted to encompass the disorder-specific molecular defects, present diagnostic modalities, and their limitations, and explored the translational possibility of Raman spectroscopy as a diagnostic tool for membranopathies.

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拉曼光谱检测红细胞膜紊乱生化变化的分析能力。

利用渗透性易脆性试验（OFT）、EMA结合试验、细胞计数法、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和下一代测序（NGS）等现有方法检测红细胞膜疾病一直具有挑战性，因为它们要么缺乏生化推断，要么本质上很复杂。拉曼光谱是一种高度分析的方法，已知可以产生分子指纹，已被证明在从单个细胞中提取生化信息方面具有潜力。利用环形激励点和线强度谱的膜靶向拉曼测量的最新进展可以将实验室芯片上拉曼激活细胞分选方法转变为一种潜在的红细胞膜疾病诊断技术。因此，我们推测，拉曼光谱可以是一个强有力的竞争者，作为诊断模式在红细胞膜病。在这篇全面的综述中，我们试图涵盖疾病特异性分子缺陷，目前的诊断方式及其局限性，并探索拉曼光谱作为膜病诊断工具的翻译可能性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Methods 生物-生化研究方法

CiteScore

9.80

自引率

2.10%

发文量

222

审稿时长

11.3 weeks

期刊介绍： Methods focuses on rapidly developing techniques in the experimental biological and medical sciences. Each topical issue, organized by a guest editor who is an expert in the area covered, consists solely of invited quality articles by specialist authors, many of them reviews. Issues are devoted to specific technical approaches with emphasis on clear detailed descriptions of protocols that allow them to be reproduced easily. The background information provided enables researchers to understand the principles underlying the methods; other helpful sections include comparisons of alternative methods giving the advantages and disadvantages of particular methods, guidance on avoiding potential pitfalls, and suggestions for troubleshooting.