Bacterial cellulose production under agitated culture conditions using tobacco waste extract by a strain of Komagataeibacter sp. isolated from rotten mango.

Abstract

Objective: This study aimed to isolate an efficient bacterial cellulose (BC)-producing strain from rotten mango, optimize BC production though single-factor tests followed by Box-Behnken design (BBD) under agitated culture conditions using tobacco waste extract as the medium, and conduct the characterization of BC via Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and X-ray diffraction (XRD).

Results: Komagataeibacter sp. XMZ1 with a high-efficiency BC biosynthesis capacity was successfully isolated from rotten mango. Subsequently, BBD was utilized to optimize the co-supplementation of lactic acid, ethanol, and yeast extract, demonstrating this statistical approach to be a reliable tool for both optimization and predictive modeling of BC yield. The optimized medium containing 2.1 g/L lactic acid, 1% (v/v) ethanol, and 2 g/L yeast extract resulted in a 2.1-fold enhancement of BC production, increasing the yield from 4.20 g/L to 8.85 g/L. The characterization of BC through FTIR, SEM and XRD revealed that BC produced under agitated culture conditions maintained similar chemical functional groups as the BC produced under static culture. However, the agitated culture products exhibited a more porous fibrillar network and a reduced crystallinity index.

Conclusion: This present study provides valuable insights into BC production under agitated culture conditions by making use of tobacco waste. Additionally, it offers BC structural characterizations that are relevant to potential industrial applications.