Ionically cross-linked pH-responsive hydrogel beads loaded with nickel cobaltite nanoparticles for controlled release of doxorubicin

IF 2.3 4区化学 Q3 CHEMISTRY, PHYSICAL

Colloid and Polymer Science Pub Date : 2025-06-11 DOI:10.1007/s00396-025-05455-y

Eppa Venkata Ramana, Guntakanti Ujwala, Thammineni Jithendra, Shaik Shahinshavali, Dandamudi Srilaxmi, Obireddy Sreekanth Reddy, Naseem

{"title":"Ionically cross-linked pH-responsive hydrogel beads loaded with nickel cobaltite nanoparticles for controlled release of doxorubicin","authors":"Eppa Venkata Ramana, Guntakanti Ujwala, Thammineni Jithendra, Shaik Shahinshavali, Dandamudi Srilaxmi, Obireddy Sreekanth Reddy,  Naseem","doi":"10.1007/s00396-025-05455-y","DOIUrl":null,"url":null,"abstract":"<div><p>Metal oxide nanoparticles are widely used in various biomedical applications due to their ability to reach unique target positions within the body. In the present work, nickel cobaltite (NiCo<sub>2</sub>O<sub>4</sub>) nanoparticles (NPs) embedded into sodium alginate/gum arabic polymeric hydrogel beads for controlled release of bioactive agents were prepared. These hydrogel beads are comprehensively examined by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM) analysis. XRD confirmed the generation of NiCo<sub>2</sub>O<sub>4</sub> NPs and integration of NPs into the hydrogel matrix. The TEM studies revealed that the size of NPs is between 25 and 30 nm. Swelling studies revealed that hydrogel beads exhibit pH-dependent behaviour, which is suitable for controlled drug release in various physiological conditions. In vitro release tests revealed a higher release rate at pH 7.4 compared to pH 2.0. Using doxorubicin as a model drug, cytotoxic effects on human breast cancer cells were examined. The developed NPs-loaded hydrogel beads effectively prevent the growth of breast cancer cells, such as Michigan Cancer Foundation-7 (MCF-7) cells with a viability of 9.5%. Furthermore, the biocompatibility of NPs and sodium alginate/gum arabic hydrogel beads conducted with 3T3 fibroblast cells has shown that cell viability is more than 80%, indicating the biocompatibility of hydrogel beads. These hydrogel beads need to warrant further development as carriers for pH-responsive and controlled release of bioactive agents.\n</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":520,"journal":{"name":"Colloid and Polymer Science","volume":"303 10","pages":"1885 - 1894"},"PeriodicalIF":2.3000,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Colloid and Polymer Science","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00396-025-05455-y","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}

引用次数: 0

Abstract

Metal oxide nanoparticles are widely used in various biomedical applications due to their ability to reach unique target positions within the body. In the present work, nickel cobaltite (NiCo₂O₄) nanoparticles (NPs) embedded into sodium alginate/gum arabic polymeric hydrogel beads for controlled release of bioactive agents were prepared. These hydrogel beads are comprehensively examined by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM) analysis. XRD confirmed the generation of NiCo₂O₄ NPs and integration of NPs into the hydrogel matrix. The TEM studies revealed that the size of NPs is between 25 and 30 nm. Swelling studies revealed that hydrogel beads exhibit pH-dependent behaviour, which is suitable for controlled drug release in various physiological conditions. In vitro release tests revealed a higher release rate at pH 7.4 compared to pH 2.0. Using doxorubicin as a model drug, cytotoxic effects on human breast cancer cells were examined. The developed NPs-loaded hydrogel beads effectively prevent the growth of breast cancer cells, such as Michigan Cancer Foundation-7 (MCF-7) cells with a viability of 9.5%. Furthermore, the biocompatibility of NPs and sodium alginate/gum arabic hydrogel beads conducted with 3T3 fibroblast cells has shown that cell viability is more than 80%, indicating the biocompatibility of hydrogel beads. These hydrogel beads need to warrant further development as carriers for pH-responsive and controlled release of bioactive agents.

Graphical Abstract

查看原文本刊更多论文

离子交联ph响应水凝胶珠载镍钴酸盐纳米颗粒控制释放阿霉素

金属氧化物纳米颗粒由于能够到达体内独特的目标位置而广泛应用于各种生物医学应用。本研究将钴酸镍（NiCo2O4）纳米颗粒包埋在海藻酸钠/阿拉伯胶聚合物水凝胶珠中，用于生物活性药物的控释。通过傅里叶变换红外光谱（FTIR）、x射线衍射（XRD）、扫描电子显微镜（SEM）和透射电子显微镜（TEM）分析对这些水凝胶珠进行了全面的检测。XRD证实了NiCo2O4 NPs的生成以及NPs在水凝胶基质中的整合。透射电镜研究表明，纳米粒子的大小在25 ~ 30 nm之间。溶胀研究表明，水凝胶珠表现出ph依赖性行为，适用于各种生理条件下的药物控释。体外释放试验显示，与pH 2.0相比，pH 7.4的释放率更高。以阿霉素为模型药物，研究了其对人乳腺癌细胞的细胞毒性作用。所开发的装载nps的水凝胶珠有效地阻止了乳腺癌细胞的生长，例如密歇根癌症基金会-7 （MCF-7）细胞的存活率为9.5%。此外，NPs和海藻酸钠/阿拉伯胶水凝胶珠与3T3成纤维细胞的生物相容性表明，细胞存活率大于80%，表明水凝胶珠具有生物相容性。这些水凝胶珠需要进一步开发作为ph响应和生物活性药物控制释放的载体。图形抽象

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Colloid and Polymer Science 化学-高分子科学

CiteScore

4.60

自引率

4.20%

发文量

111

审稿时长

2.2 months

期刊介绍： Colloid and Polymer Science - a leading international journal of longstanding tradition - is devoted to colloid and polymer science and its interdisciplinary interactions. As such, it responds to a demand which has lost none of its actuality as revealed in the trends of contemporary materials science.