Occurrence and risk assessment of tetracycline residues in poultry meat of Dhaka, Bangladesh: A sensitive and reliable analytical method development approach

Food chemistry advances Pub Date : 2025-09-19 DOI:10.1016/j.focha.2025.101120

Tajnin Jahan , Sabina Yasmin , Mohammad Saydur Rahman , Bakiya Begum Tabassum , Sharmin Akter Lisa , Md. Alamgir Kabir , Md Humayun Kabir

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Abstract

This study presents a modified method for quantifying tetracycline antibiotics (tetracycline, oxytetracycline, chlortetracycline, and doxycycline) in poultry meat based on the analysis of 40 poultry samples collected from local markets in Bangladesh. The method employed liquid chromatography-tandem mass spectrometry (LC-MS/MS) with methanol extraction and optimized dispersive solid phase extraction (d-SPE) cleanup (using C18, GCB, and PSA). Matrix-matched calibration curves (r² ≥ 0.997) and validation at 10, 50, and 100 µg/kg spiking levels yielded recoveries of 82.6-116.5 % with RSDs ≤ 8.9 %. The method achieved low LODs (1.67-3.33 µg/kg) and LOQs (5-10 µg/kg). The results from the analysis of 40 poultry samples revealed that 55 % were contaminated with tetracycline residues (7.60–144.98 µg/kg), with oxytetracycline being the most prevalent. Hazard indices (HIs) indicated negligible health risks, although liver samples posed higher risks. The findings support regulatory measures to control antibiotic use in poultry farming in Bangladesh.

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孟加拉国达卡禽肉中四环素残留的发生和风险评估：一种敏感可靠的分析方法开发方法

本研究基于对孟加拉国当地市场收集的40个家禽样本的分析，提出了一种改进的禽肉中四环素类抗生素（四环素、土霉素、金四素和多西环素）定量方法。该方法采用液相色谱-串联质谱法（LC-MS/MS）甲醇提取和优化分散固相萃取（d-SPE）净化（C18、GCB和PSA）。矩阵匹配校准曲线（r²≥0.997），在10、50和100µg/kg添加水平下验证，回收率为82.6 ~ 116.5%,rsd≤8.9%。该方法检出限低（1.67 ~ 3.33µg/kg），检出限低（5 ~ 10µg/kg）。对40份家禽样本的分析结果显示，55%的家禽样本被四环素残留污染（7.60 ~ 144.98µg/kg），其中土霉素污染最为普遍。危害指数（HIs）表明，健康风险可以忽略不计，但肝脏样本构成的风险更高。研究结果支持在孟加拉国控制家禽养殖中抗生素使用的监管措施。

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