A ratiometric fluorescent paper–based sensor based on Fe/Eu-MOF for visual and sensitive detection of diethylstilbestrol

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2025-09-20 DOI:10.1016/j.bios.2025.118017

Dongmei Liu , Xinhui Jiang , Xiaoming Li , Hecun Zou , Lin He , Zhengwei Zhang , Siqiao Li , Xintong Bian

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引用次数: 0

Abstract

Diethylstilbestrol (DES), a synthetic estrogen, is associated with severe endocrine disruption and carcinogenic risks, posing persistent biological and environmental hazards. Herein, a bimetallic Fe/Eu–based metal–organic framework (Fe/Eu–MOF) with distinct multi–emission properties was developed as a ratiometric fluorescent sensor for sensitive and intelligent visual detection of DES. The Fe/Eu–MOF exhibited stable red emission at 615 nm and DES–responsive blue emission at 410 nm, enabling self–referenced quantification. The sensor showed two linear ranges (0.005–2 and 2–30 μg/mL), a low detection limit (1.78 ng/mL), and excellent selectivity against potential interferents. Its practicality was validated in milk and shrimp, demonstrating excellent recovery and reproducibility. Additionally, a paper–based sensor integrated with smartphone RGB analysis enabled rapid, on–site, and instrument–free detection. Mechanism studies revealed that DES coordination with Fe centers suppressed non–radiative decay and enhanced fluorescence. This work provides a robust and intelligent platform for portable monitoring of DES residues, offering significant potential for real–time biohazard surveillance and visual diagnostics in resource–limited settings.

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基于Fe/Eu-MOF的比例荧光纸型传感器用于二乙基己烯雌酚的视觉灵敏检测

己烯雌酚（DES）是一种合成雌激素，具有严重的内分泌干扰和致癌风险，对生物和环境造成持续危害。本文开发了一种具有不同多发射特性的双金属Fe/ eu基金属有机骨架（Fe/ Eu-MOF）作为比例荧光传感器，用于DES的灵敏和智能视觉检测。Fe/ Eu-MOF在615 nm处具有稳定的红色发射，在410 nm处具有DES响应的蓝色发射，可以进行自参定量。该传感器具有0.005-2和2-30 μg/mL两个线性范围，检出限低（1.78 ng/mL），对潜在干扰具有良好的选择性。在牛奶和对虾中验证了该方法的实用性，具有良好的回收率和重复性。此外，基于纸张的传感器与智能手机的RGB分析集成在一起，实现了快速、现场和无仪器的检测。机理研究表明，DES与Fe中心配位抑制了非辐射衰变，增强了荧光。这项工作为便携式监测DES残留提供了一个强大的智能平台，为资源有限的环境下的实时生物危害监测和视觉诊断提供了巨大的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.