Viviana Cristina Peixoto , Iliseu Monteiro Alcântara , Heloisa Aparecida Silva de Assis , Marcus Vinicius Astolfo da Costa , Caio Roberto Soares Bragança , Marita Gimenez Pereira , Sarah Regina Vargas
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引用次数: 0
Abstract
Bioethanol is a renewable energy alternative and can be produced from biomass rich in organic matter such as coffee straw, an abundant agro-industrial waste in Brazil. This study evaluated fermentative bioprocesses with two strains of wild yeasts and using hydrolysate obtained in the treatment of coffee straw by thermochemical hydrolysis in batch systems for obtaining second-generation ethanol. The molecular identification of 15 strains of wild yeasts isolated from brewery wastewater and decaying fruit was performed. A screening of those strains revealed Pichia kudriavzevii (LMP-Y 10) and Saccharomyces cerevisiae (LMP-Y 14) showed the best ethanol production (1.64 and 1.91 g L−1, respectively). According to the optimization of biomass production, LMP-Y 10 at 38 °C, 20 g L−1 of glucose, and pH 6.0 and LMP-Y 14, at 34 °C, 30 g L−1 of glucose, and pH 7.0 provided better biomass yield. Fermentation bioprocess assays in a batch system from hydrolyzed coffee husk pretreated with H2SO4 1 % and 2 %, with and without agitation, led to better ethanol production with H2SO4 2 %, obtaining 0.58 g L−1 with LMP-Y 10, under agitation, and 0.43 g L−1 with LMP-Y 14, in static condition for both strains (in 20 mL of cultures at an F/M ratio = 1). The results reveal two previously unstudied wild strains with strong potential for second-generation bioethanol production through the valorization of coffee husk that can contribute to future research and advances in biotechnology, offering sustainable alternatives to produce bioenergy from agro-industrial waste.
期刊介绍:
Biomass & Bioenergy is an international journal publishing original research papers and short communications, review articles and case studies on biological resources, chemical and biological processes, and biomass products for new renewable sources of energy and materials.
The scope of the journal extends to the environmental, management and economic aspects of biomass and bioenergy.
Key areas covered by the journal:
• Biomass: sources, energy crop production processes, genetic improvements, composition. Please note that research on these biomass subjects must be linked directly to bioenergy generation.
• Biological Residues: residues/rests from agricultural production, forestry and plantations (palm, sugar etc), processing industries, and municipal sources (MSW). Papers on the use of biomass residues through innovative processes/technological novelty and/or consideration of feedstock/system sustainability (or unsustainability) are welcomed. However waste treatment processes and pollution control or mitigation which are only tangentially related to bioenergy are not in the scope of the journal, as they are more suited to publications in the environmental arena. Papers that describe conventional waste streams (ie well described in existing literature) that do not empirically address ''new'' added value from the process are not suitable for submission to the journal.
• Bioenergy Processes: fermentations, thermochemical conversions, liquid and gaseous fuels, and petrochemical substitutes
• Bioenergy Utilization: direct combustion, gasification, electricity production, chemical processes, and by-product remediation
• Biomass and the Environment: carbon cycle, the net energy efficiency of bioenergy systems, assessment of sustainability, and biodiversity issues.