Occupational exposure to pesticides reveals a miRNAs expression profile indicating multiple disease risks

IF 6.1 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Ecotoxicology and Environmental Safety Pub Date : 2025-09-23 DOI:10.1016/j.ecoenv.2025.119065

Luiza Flavia Veiga Francisco , Willian Garcia Birolli , Welinton Hirai , Ana Julia Aguiar de Freitas , Paula Rohr , Caroline Rocha Nunes , Álvaro José dos Santos Neto , Fernando Barbosa Junior , Márcia Maria Chiquitelli Marques , Henrique C.S. Silveira

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引用次数: 0

Abstract

Growing evidence links pesticide exposure to health issues, including pulmonary, cardiovascular, neurodegenerative diseases, and cancer. This study analyzed microRNA (miRNAs) expression in pesticide-exposed individuals and predicted the biological functions of these miRNAs through target gene analysis. A cohort of 23 pesticide applicators and 23 non-exposed individuals were recruited in Brazil. Serum pesticide quantification revealed a significant difference in the concentration of dichlorodiphenyldichloroethylene (DDE) between the two groups. Cellular analysis revealed increased frequencies of micronucleated, binucleated, pyknotic, and karyolytic cells in the exposed group. Plasma analysis identified 30 differentially expressed miRNAs, of which 20 were selected for further study based on receiver operating characteristic (ROC) curve analysis (AUC ≥ 0.75, p < 0.005). These miRNAs targeted a total of 1004 validated target genes. The application of functional analysis yielded a total of 213 biological processes, 131 cellular components, and 170 molecular functions. Furthermore, 91 enriched pathways and 107 diseases with the most prevalent being neurological disorders, arthritis, and cancer, were associated with the predicted target genes. This study reveals biological pathways affected by pesticide exposure and highlights the critical role of miRNAs in disease pathogenesis, particularly for neurological conditions and cancers.

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职业性农药暴露揭示了mirna表达谱，表明多种疾病风险

越来越多的证据表明，农药暴露与健康问题有关，包括肺病、心血管病、神经退行性疾病和癌症。本研究分析了农药暴露个体中microRNA （miRNAs）的表达，并通过靶基因分析预测了这些mirna的生物学功能。在巴西招募了23名农药施用者和23名未接触者。血清农药定量显示两组间二氯二苯二氯乙烯（DDE）浓度有显著差异。细胞分析显示，暴露组微核、双核、缩缩和核溶解细胞的频率增加。血浆分析鉴定出30个差异表达的mirna，根据受试者工作特征（ROC）曲线分析（AUC≥0.75,p <； 0.005），从中选择20个进行进一步研究。这些mirna总共靶向1004个经过验证的靶基因。功能分析一共得到213个生物过程、131个细胞组分和170个分子功能。此外，91个富集通路和107种疾病与预测的靶基因相关，其中最常见的是神经系统疾病、关节炎和癌症。这项研究揭示了受农药暴露影响的生物学途径，并强调了mirna在疾病发病机制中的关键作用，特别是在神经系统疾病和癌症中。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ecotoxicology and Environmental Safety 环境科学-毒理学

CiteScore

12.10

自引率

5.90%

发文量

1234

审稿时长

88 days

期刊介绍： Ecotoxicology and Environmental Safety is a multi-disciplinary journal that focuses on understanding the exposure and effects of environmental contamination on organisms including human health. The scope of the journal covers three main themes. The topics within these themes, indicated below, include (but are not limited to) the following: Ecotoxicology、Environmental Chemistry、Environmental Safety etc.