Matrix influence of collagen: fibrin interpenetrating hydrogels on microvascular networks and osteogenesis

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications Pub Date : 2025-09-19 DOI:10.1016/j.bioadv.2025.214518

Gennifer Chiou , Sarah Stagg , Gabriela Gonzales , Liliana Danford , Isaiah Arredondo , Rena Bizios , Joo L. Ong , Teja Guda

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引用次数: 0

Abstract

Blended interpenetrating collagen: fibrin (col: fib) blend hydrogels of differing blend ratios (100:0, 25:75, 50:50, 75:25, and 0:100 col.:fib respectively) were synthesized for the purposes of supporting angiogenesis and osteogenesis. Gels were seeded with microvascular fragments (MVFs) to evaluate how the col.: fib matrix alone supported angiogenesis. Gels were also seeded with mesenchymal stem cells (MSCs) in conjunction with the MVFs to determine how stromal support in addition to the matrix supported both angiogenesis and osteogenesis, with stromal conditioning from osteogenic differentiation media. Gel blends with higher amounts of fibrin content supported angiogenesis of MVF seeded samples better with increased levels of vessel network formation and sprouting. When stromal cell support was added, this trend remained, with evidence that stromal cells aided in faster and more robust vessel formation. Gene expression levels indicate that the 50C50F blend balances osteogenic and angiogenic support. Altogether, we found that matrix composition dictates vessel infiltration and spreading and that with the addition of stromal cells, both angio- and osteogenesis were accelerated. The results of this study demonstrate that a blend ratio of 50:50 col.:fib balances angiogenesis and osteogenesis within a co-culture of MSCs and MVFs.

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胶原-纤维蛋白互穿水凝胶对微血管网络和成骨的基质影响

合成了不同混合比例（分别为100:0,25:75,50:50,75:25和0:100 col.:fib）的混合互穿胶原蛋白：纤维蛋白（col: fib）混合水凝胶，用于支持血管生成和骨生成。在凝胶中植入微血管碎片（MVFs），以评估col. fib基质单独支持血管生成的效果。凝胶中还植入了间充质干细胞（MSCs）和MVFs，以确定基质支持如何支持血管生成和成骨生成，基质调节来自成骨分化培养基。纤维蛋白含量较高的凝胶混合物更好地支持MVF种子样品的血管生成，血管网络形成和发芽水平增加。当添加基质细胞支持时，这种趋势仍然存在，有证据表明基质细胞有助于更快、更强健的血管形成。基因表达水平表明50C50F混合物平衡了成骨和血管生成的支持。总之，我们发现基质成分决定了血管的浸润和扩张，并且随着基质细胞的加入，血管和骨的形成都加快了。本研究结果表明，在MSCs和MVFs共培养中，50:50的col.:fib混合比例可以平衡血管生成和成骨生成。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Materials Science & Engineering C-Materials for Biological Applications 工程技术-材料科学：生物材料

CiteScore

17.80

自引率

0.00%

发文量

501

审稿时长

27 days

期刊介绍： Biomaterials Advances, previously known as Materials Science and Engineering: C-Materials for Biological Applications (P-ISSN: 0928-4931, E-ISSN: 1873-0191). Includes topics at the interface of the biomedical sciences and materials engineering. These topics include: • Bioinspired and biomimetic materials for medical applications • Materials of biological origin for medical applications • Materials for "active" medical applications • Self-assembling and self-healing materials for medical applications • "Smart" (i.e., stimulus-response) materials for medical applications • Ceramic, metallic, polymeric, and composite materials for medical applications • Materials for in vivo sensing • Materials for in vivo imaging • Materials for delivery of pharmacologic agents and vaccines • Novel approaches for characterizing and modeling materials for medical applications Manuscripts on biological topics without a materials science component, or manuscripts on materials science without biological applications, will not be considered for publication in Materials Science and Engineering C. New submissions are first assessed for language, scope and originality (plagiarism check) and can be desk rejected before review if they need English language improvements, are out of scope or present excessive duplication with published sources. Biomaterials Advances sits within Elsevier''s biomaterials science portfolio alongside Biomaterials, Materials Today Bio and Biomaterials and Biosystems. As part of the broader Materials Today family, Biomaterials Advances offers authors rigorous peer review, rapid decisions, and high visibility. We look forward to receiving your submissions!