A tyrosinase-functionalized graphene Nanofiber/AuNP biosensor for clinical detection of alkaline phosphatase

Abstract

A novel graphene nanofiber-based electrochemical biosensing strategy was developed for ultrasensitive and specific ALP detection using the tyrosinase enzyme. Gold nanoparticles decorated GNFs were utilized as a signal amplification platform for electrochemical detection. GNFs/AuNPs/Tyrosinase modified PG surface utilized as a catalytic amplification platform for ALP activity determination. ALP act as biomarker for wide range of clinical conditions specially, liver and bone disorders. The optimum pH, temperature, and phenyl phosphate substrate concentration for the fabricated sensor were 8, 30 °C, and 0.1 mM, respectively. The proposed biosensor exhibits excellent selectivity and sensitivity with a dynamic linear range from 1 to 2200U/L with a detection limit of 1U/L, along with better reproducibility. Human serum samples of healthy individuals and liver patients were successfully analyzed for ALP detection with good results via a simple and rapid approach.