Dual-function fluorescent sensor enabling real-time tracking of cellular viscosity and biothiols levels

IF 4.6 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-09-19 DOI:10.1016/j.saa.2025.126967

Xiaomeng Pang , Siyuan Qin , Hailong Ma , Yanbi Luo , Qiang Fei , Su Xu , Feiyi Wang

引用次数: 0

Abstract

Biothiols, as a biomarker, play an important role in regulating physiological metabolism and maintaining redox homeostasis. Viscosity, as a microenvironment factor of cells, is closely related to many human diseases. As a powerful tool, fluorescent probes have been widely used in the detection of biomarkers. In this paper, a dual-function fluorescent probe IDH-NBD integrating real-time detection of viscosity and biothiols levels was constructed. The probe showed good response efficiency and sensitivity to biothiols and viscosity in vitro, and the preliminary mechanism of action was also explored. Further biological imaging experiments also showed that the probe could monitor the biothiols levels and viscosity of cells and zebrafish. Here, we provided a new tool for the prevention and early diagnosis of related diseases in organisms.

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双功能荧光传感器，实时跟踪细胞粘度和生物硫醇水平

生物硫醇作为一种生物标志物，在调节生理代谢和维持氧化还原稳态中起着重要作用。黏度作为细胞的微环境因子，与人类许多疾病密切相关。荧光探针作为一种强有力的工具，已广泛应用于生物标志物的检测。本文构建了一种实时检测黏度和生物硫醇水平的双功能荧光探针IDH-NBD。该探针在体外对生物硫醇和黏度具有良好的响应效率和敏感性，并对其作用机制进行了初步探讨。进一步的生物成像实验也表明，该探针可以监测细胞和斑马鱼的生物硫醇水平和粘度。本研究为生物体内相关疾病的预防和早期诊断提供了新的工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.