Ultrafast photonic qPCR system based on 2D photothermal black phosphorus for African Swine Fever virus (ASFV) detection

IF 3.9 2区材料科学 Q2 MATERIALS SCIENCE, MULTIDISCIPLINARY

Vacuum Pub Date : 2025-09-23 DOI:10.1016/j.vacuum.2025.114771

Zhixian Liang , Shiping Zhou , Weikang Xu, Shaohui Zhang

引用次数: 0

Abstract

We have innovatively developed microfluidic chips utilizing the 2D black phosphorus (BP) as a photothermal medium, and the reaction chambers of microfluidic systems based on PDMS-coated BP nanosheets (BPNs) were crafted as molded elastomers. Furthermore, the chip chamber structure necessitates merely 4 μL of reagents, a significant reduction compared to the volumes required by traditional PCR tubes. Additionally, the superior photothermal conversion efficiency of BPNs facilitates the attainment of PCR amplification temperatures, enabling the entire PCR thermal cycle (45 cycles) to be completed for detecting African Swine Fever virus (ASFV) within 25 min. Crucially, this method opens up new avenues for the application of BPNs within photothermal PCR detection systems, offering a valuable rapid assay with the potential for immediate diagnosis of nucleic acid viruses.

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基于二维光热黑磷的超快光子qPCR检测非洲猪瘟病毒

我们创新地开发了利用二维黑磷（BP）作为光热介质的微流控芯片，并将基于pdms涂层BP纳米片（BPNs）的微流控系统的反应室制成模压弹性体。此外，芯片腔结构只需要4 μL的试剂，与传统PCR管所需的体积相比显着减少。此外，bpn优越的光热转换效率有助于获得PCR扩增温度，使整个PCR热循环（45个循环）在25 min内完成检测非洲猪瘟病毒（ASFV）。至关重要的是，该方法为bpn在光热PCR检测系统中的应用开辟了新的途径，提供了一种有价值的快速检测方法，具有立即诊断核酸病毒的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Vacuum 工程技术-材料科学：综合

CiteScore

6.80

自引率

17.50%

发文量

审稿时长

34 days

期刊介绍： Vacuum is an international rapid publications journal with a focus on short communication. All papers are peer-reviewed, with the review process for short communication geared towards very fast turnaround times. The journal also published full research papers, thematic issues and selected papers from leading conferences. A report in Vacuum should represent a major advance in an area that involves a controlled environment at pressures of one atmosphere or below. The scope of the journal includes: 1. Vacuum; original developments in vacuum pumping and instrumentation, vacuum measurement, vacuum gas dynamics, gas-surface interactions, surface treatment for UHV applications and low outgassing, vacuum melting, sintering, and vacuum metrology. Technology and solutions for large-scale facilities (e.g., particle accelerators and fusion devices). New instrumentation ( e.g., detectors and electron microscopes). 2. Plasma science; advances in PVD, CVD, plasma-assisted CVD, ion sources, deposition processes and analysis. 3. Surface science; surface engineering, surface chemistry, surface analysis, crystal growth, ion-surface interactions and etching, nanometer-scale processing, surface modification. 4. Materials science; novel functional or structural materials. Metals, ceramics, and polymers. Experiments, simulations, and modelling for understanding structure-property relationships. Thin films and coatings. Nanostructures and ion implantation.