Nafion-stabilized silver nanoparticles modified glassy carbon electrode for ultrasensitive detection of alpha-1-acid glycoprotein

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry Pub Date : 2025-09-19 DOI:10.1016/j.bioelechem.2025.109112

Gulam Rabbani , Akbar Mohammad , Mohammad Ehtisham Khan , Waleed Zakri , Mohsin Vahid Khan , Khurshid Ahamd , Wahid Ali , Syed Kashif Ali , Nazim Hasan , Abdulrahman Khamaj , Jintae Lee

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Abstract

In this study, alpha-1-acid glycoprotein (AGP), a crucial biomarker associated with various diseases and physiological conditions, was selected for detection using the developed immunosensor. The immunosensor was fabricated by depositing a synthesized Nafion/silver nanoparticles (AgNPs) nanocomposite on the glassy carbon electrode as the substrate material. The changes in the physicochemical properties after Nafion/AgNPs nanocomposite deposition were analyzed through SEM and XPS measurements. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to analyze the immunosensor fabrication's electrochemical performance. AGP was detected through the immobilized anti-AGP on the electrode surface. The developed immunosensor exhibited a wide linear detection range from 0.05 to 3.2 mg/mL and a LOD of 0.17 mg/mL. The developed immunosensor showed excellent selectivity and repeatability when subjected to different kinds of interfering proteins. The recovery of AGP ranged from 101.7 to 103.7 % and RSD was <4 %, indicating high accuracy in real samples detection. The immunosensor developed for AGP detection showed a number of beneficial characteristics, including low cost and small volume sample requirements, making it highly suitable for point-of-care applications. This study provides new insights into the precise fabrication and affordable immunosensors for diverse clinical diagnostic applications.

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钠稳定的纳米银修饰玻碳电极用于α -1-酸性糖蛋白的超灵敏检测

本研究选择与多种疾病和生理状况相关的重要生物标志物α -1-酸性糖蛋白（AGP）进行检测。将合成的纳米银纳米复合材料（AgNPs）沉积在玻碳电极上作为衬底材料制备免疫传感器。通过SEM和XPS测试分析了纳米复合材料沉积后理化性质的变化。利用循环伏安法（CV）和电化学阻抗谱法（EIS）分析了免疫传感器的电化学性能。AGP通过固定在电极表面的抗AGP检测。该免疫传感器的线性检测范围为0.05 ~ 3.2 mg/mL， LOD为0.17 mg/mL。所研制的免疫传感器对不同种类的干扰蛋白具有良好的选择性和重复性。AGP的回收率为101.7% ~ 103.7%，RSD为4%，对实际样品的检测具有较高的准确性。用于AGP检测的免疫传感器显示出许多有益的特性，包括低成本和小体积样品要求，使其非常适合于护理点应用。这项研究为精确制造和负担得起的免疫传感器提供了新的见解，用于各种临床诊断应用。

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.