Bolu Sun , Haiying He , Zixia Wang , Yuqiong Wu , Jiali Kang , Xinlan Wang , Xuanxiu Da , Miao Zhou , Ying Lv , Chenyu Qin , Xiangdong Wang , Lin Yang , Huajie Li , Hai Wang
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引用次数: 0
Abstract
Cancer chemotherapy, while enhancing patient survival, is limited by cardiotoxicity. Early monitoring and risk assessment are essential. C-reactive protein (CRP) can serve as an early biomarker for predicting cardiotoxicity during chemotherapy, offering a critical opportunity to develop new cardioprotective strategies. This study used an anti-CRP antibody loaded onto an electrode modified with chitosan (CS) and functionalized carbon black (f-CB) as a target. Due to f-CB's excellent electrical conductivity and CS's high permeability and strong adhesion, an immunosensor for the early monitoring and risk assessment of chemotherapy-induced cardiotoxicity based on CRP-specific immunorecognition was successfully constructed. Under optimal experimental conditions, the immunosensor exhibited excellent linearity in the range of 3.9 × 10−1 to 4 × 102 ng mL−1 with a detection limit as low as 3.16 × 10−1 ng mL−1, making it more sensitive than ELISA. The immunosensor also demonstrated excellent selectivity, reproducibility, and stability in detecting CRP in a chemotherapy-induced cardiotoxicity model, with recovery rates ranging from 97.14 % to 107.65 %. This study presents an efficient, accurate, and practical approach for the early detection and risk assessment of chemotherapy-induced cardiotoxicity, thereby supporting a balance between therapeutic efficacy and cardiac safety.
期刊介绍:
An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry
Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of:
• Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction.
• Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms)
• Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes)
• Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion)
• Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair).
• Organization and use of arrays in-vitro and in-vivo, including as part of feedback control.
• Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.