Sustainable valorization of treated wastewater with the green microalga Monoraphidium minutum for valuable products

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts Pub Date : 2025-09-22 DOI:10.1016/j.algal.2025.104319

Zakaria Tazart , Ranya Saidi , Abdallah Oukarroum

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Abstract

This study evaluates the potential of Monoraphidium minutum, a microalgal strain isolated from a Moroccan coastal wetland, for biomass production and wastewater treatment. Unlike previous studies, this research explores the growth of the organism in treated wastewater (TWW) at varying dilution levels, assessing its role in nutrient removal and biochemical accumulation. Experimental cultures were conducted over ten days in BG11 (control), 100 % TWW, 100 % seawater (SW), and three TWW-SW mixtures (75 %, 50 %, 25 %). Growth, biochemical composition (lipids, proteins, pigments), photosynthetic efficiency, and nutrient removal were analyzed. Results show that 100 % TWW supported the highest biomass (140 × 10⁵ cells/mL in flasks, 200 × 10⁵ cells/mL in a photobioreactor). Lipid content peaked at ~30 %, with a fatty acid profile rich in palmitic and oleic acids, emphasizing its biofuel potential. Carotenoid accumulation and photosynthetic efficiency were highest in 100 % TWW. Nutrient removal exceeded 96 % for nitrogen and 98 % for phosphorus. These findings confirm the feasibility of M. minutum for wastewater bioremediation and bioresource recovery, positioning TWW as a sustainable and cost-effective medium for microalgae cultivation and biofuel production.

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绿色微藻对处理过的废水的可持续增值，以获得有价产品

本研究评估了一种从摩洛哥沿海湿地分离出来的微藻菌株Monoraphidium minutum在生物质生产和废水处理方面的潜力。与以往的研究不同，本研究探索了不同稀释水平下处理废水（TWW）中生物的生长，评估了其在营养去除和生化积累中的作用。在BG11（对照）、100% TWW、100%海水（SW）和三种TWW-SW混合物（75%、50%、25%）中进行10天的实验培养。分析了生长、生化组成（脂质、蛋白质、色素）、光合效率和营养去除。结果表明，100% TWW的生物量最高（烧瓶中140 × 105个细胞/mL，光生物反应器中200 × 105个细胞/mL）。脂质含量最高达30%，脂肪酸谱富含棕榈酸和油酸，强调了其生物燃料潜力。类胡萝卜素积累和光合效率在100% TWW时最高。氮和磷的去除率分别超过96%和98%。这些发现证实了M. minutum用于废水生物修复和生物资源回收的可行性，将TWW定位为微藻培养和生物燃料生产的可持续且具有成本效益的培养基。

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来源期刊

Algal Research-Biomass Biofuels and Bioproducts BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

9.40

自引率

7.80%

发文量

332

期刊介绍： Algal Research is an international phycology journal covering all areas of emerging technologies in algae biology, biomass production, cultivation, harvesting, extraction, bioproducts, biorefinery, engineering, and econometrics. Algae is defined to include cyanobacteria, microalgae, and protists and symbionts of interest in biotechnology. The journal publishes original research and reviews for the following scope: algal biology, including but not exclusive to: phylogeny, biodiversity, molecular traits, metabolic regulation, and genetic engineering, algal cultivation, e.g. phototrophic systems, heterotrophic systems, and mixotrophic systems, algal harvesting and extraction systems, biotechnology to convert algal biomass and components into biofuels and bioproducts, e.g., nutraceuticals, pharmaceuticals, animal feed, plastics, etc. algal products and their economic assessment