Comparative clinical characterization of microflora between primary and secondary endodontic infections-An in vivo cross sectional study

Q1 Medicine
Mahantesh Yeli , Balaram Naik , RaghavendraD. Kulkarni , Vignesh Kamath , Prashant Moogi , Shruti Patil , Kishore Bhat
{"title":"Comparative clinical characterization of microflora between primary and secondary endodontic infections-An in vivo cross sectional study","authors":"Mahantesh Yeli ,&nbsp;Balaram Naik ,&nbsp;RaghavendraD. Kulkarni ,&nbsp;Vignesh Kamath ,&nbsp;Prashant Moogi ,&nbsp;Shruti Patil ,&nbsp;Kishore Bhat","doi":"10.1016/j.jobcr.2025.09.018","DOIUrl":null,"url":null,"abstract":"<div><h3>Aim</h3><div>The aim of the present study was to analyse the microbiota of primary and secondary/persistent endodontic infections of patients undergoing endodontic treatment with respect to clinical and radiographic findings.</div></div><div><h3>Materials and methodology</h3><div>The experimental material included the samples collected from 139 patients. These subjects were divided into two groups of 74 and 65 patients. Group 1-Primary endodontic infections, Group 2-Secondary endodontic infections (Retreatment cases). The collected samples were subjected to aerobic and anaerobic bacterial cultures and selected specific uncultivable microorganisms were subjected to Polymerase Chain Reaction. The material collected in transport medium was mixed thoroughly and it was divided into two aliquots. One of the aliquots was used for aerobic culture and Polymerase Chain Reaction, the other was subjected to anaerobic culture study. Statistical analysis was done. P &lt; 0.05 was considered as statistically significant.</div></div><div><h3>Results</h3><div>For the given sample, the values for Porphyromonas gingivalis (p = 0.001), Prevotella intermedia (p = 0.017) and Streptococcus aerobic (p = 0.001), Klebsiella aerogens (p = 0.03) and Streptococcus (p = 0.005) and Dialister invisus (p = 0.024) showed statistically significant differences between primary and secondary lesions.</div></div><div><h3>Conclusions</h3><div>The primary lesions showed significantly higher number of Klebsiella aerogens, Streptococcus, Dialister invisus and secondary lesions with Porphyromonas gingivalis, Prevotella intermedia, Streptococcus respectively.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1579-1583"},"PeriodicalIF":0.0000,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of oral biology and craniofacial research","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2212426825002313","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0

Abstract

Aim

The aim of the present study was to analyse the microbiota of primary and secondary/persistent endodontic infections of patients undergoing endodontic treatment with respect to clinical and radiographic findings.

Materials and methodology

The experimental material included the samples collected from 139 patients. These subjects were divided into two groups of 74 and 65 patients. Group 1-Primary endodontic infections, Group 2-Secondary endodontic infections (Retreatment cases). The collected samples were subjected to aerobic and anaerobic bacterial cultures and selected specific uncultivable microorganisms were subjected to Polymerase Chain Reaction. The material collected in transport medium was mixed thoroughly and it was divided into two aliquots. One of the aliquots was used for aerobic culture and Polymerase Chain Reaction, the other was subjected to anaerobic culture study. Statistical analysis was done. P < 0.05 was considered as statistically significant.

Results

For the given sample, the values for Porphyromonas gingivalis (p = 0.001), Prevotella intermedia (p = 0.017) and Streptococcus aerobic (p = 0.001), Klebsiella aerogens (p = 0.03) and Streptococcus (p = 0.005) and Dialister invisus (p = 0.024) showed statistically significant differences between primary and secondary lesions.

Conclusions

The primary lesions showed significantly higher number of Klebsiella aerogens, Streptococcus, Dialister invisus and secondary lesions with Porphyromonas gingivalis, Prevotella intermedia, Streptococcus respectively.

Abstract Image

原发性和继发性牙髓感染的微生物群临床特征比较-一项体内横断面研究
目的本研究的目的是分析接受根管治疗的患者的原发性和继发性/持续性根管感染的微生物群,以及临床和放射学表现。材料和方法实验材料包括139例患者的样本。这些受试者被分为两组,每组74名和65名患者。组1-原发性牙髓感染,组2-继发性牙髓感染(再治疗病例)。收集的样品进行好氧和厌氧细菌培养,并选择特定的不可培养微生物进行聚合酶链反应。将收集到的物料在输送介质中充分混合,并分成两等分。其中一份用于好氧培养和聚合酶链反应,另一份用于厌氧培养研究。进行统计分析。P <; 0.05认为有统计学意义。结果原发性和继发性病变中牙龈卟啉单胞菌(p = 0.001)、中间普雷沃菌(p = 0.017)、需氧链球菌(p = 0.001)、克雷伯菌(p = 0.03)、链球菌(p = 0.005)和内溶Dialister (p = 0.024)的检出率差异均有统计学意义。结论原发性病变中肺克雷伯菌、链球菌、内隐Dialister数量显著高于原发性病变,继发性病变中分别有牙龈卟啉单胞菌、中间普雷沃菌、链球菌。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
4.90
自引率
0.00%
发文量
133
审稿时长
167 days
期刊介绍: Journal of Oral Biology and Craniofacial Research (JOBCR)is the official journal of the Craniofacial Research Foundation (CRF). The journal aims to provide a common platform for both clinical and translational research and to promote interdisciplinary sciences in craniofacial region. JOBCR publishes content that includes diseases, injuries and defects in the head, neck, face, jaws and the hard and soft tissues of the mouth and jaws and face region; diagnosis and medical management of diseases specific to the orofacial tissues and of oral manifestations of systemic diseases; studies on identifying populations at risk of oral disease or in need of specific care, and comparing regional, environmental, social, and access similarities and differences in dental care between populations; diseases of the mouth and related structures like salivary glands, temporomandibular joints, facial muscles and perioral skin; biomedical engineering, tissue engineering and stem cells. The journal publishes reviews, commentaries, peer-reviewed original research articles, short communication, and case reports.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信