{"title":"Comparative clinical characterization of microflora between primary and secondary endodontic infections-An in vivo cross sectional study","authors":"Mahantesh Yeli , Balaram Naik , RaghavendraD. Kulkarni , Vignesh Kamath , Prashant Moogi , Shruti Patil , Kishore Bhat","doi":"10.1016/j.jobcr.2025.09.018","DOIUrl":null,"url":null,"abstract":"<div><h3>Aim</h3><div>The aim of the present study was to analyse the microbiota of primary and secondary/persistent endodontic infections of patients undergoing endodontic treatment with respect to clinical and radiographic findings.</div></div><div><h3>Materials and methodology</h3><div>The experimental material included the samples collected from 139 patients. These subjects were divided into two groups of 74 and 65 patients. Group 1-Primary endodontic infections, Group 2-Secondary endodontic infections (Retreatment cases). The collected samples were subjected to aerobic and anaerobic bacterial cultures and selected specific uncultivable microorganisms were subjected to Polymerase Chain Reaction. The material collected in transport medium was mixed thoroughly and it was divided into two aliquots. One of the aliquots was used for aerobic culture and Polymerase Chain Reaction, the other was subjected to anaerobic culture study. Statistical analysis was done. P < 0.05 was considered as statistically significant.</div></div><div><h3>Results</h3><div>For the given sample, the values for Porphyromonas gingivalis (p = 0.001), Prevotella intermedia (p = 0.017) and Streptococcus aerobic (p = 0.001), Klebsiella aerogens (p = 0.03) and Streptococcus (p = 0.005) and Dialister invisus (p = 0.024) showed statistically significant differences between primary and secondary lesions.</div></div><div><h3>Conclusions</h3><div>The primary lesions showed significantly higher number of Klebsiella aerogens, Streptococcus, Dialister invisus and secondary lesions with Porphyromonas gingivalis, Prevotella intermedia, Streptococcus respectively.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1579-1583"},"PeriodicalIF":0.0000,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of oral biology and craniofacial research","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2212426825002313","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0
Abstract
Aim
The aim of the present study was to analyse the microbiota of primary and secondary/persistent endodontic infections of patients undergoing endodontic treatment with respect to clinical and radiographic findings.
Materials and methodology
The experimental material included the samples collected from 139 patients. These subjects were divided into two groups of 74 and 65 patients. Group 1-Primary endodontic infections, Group 2-Secondary endodontic infections (Retreatment cases). The collected samples were subjected to aerobic and anaerobic bacterial cultures and selected specific uncultivable microorganisms were subjected to Polymerase Chain Reaction. The material collected in transport medium was mixed thoroughly and it was divided into two aliquots. One of the aliquots was used for aerobic culture and Polymerase Chain Reaction, the other was subjected to anaerobic culture study. Statistical analysis was done. P < 0.05 was considered as statistically significant.
Results
For the given sample, the values for Porphyromonas gingivalis (p = 0.001), Prevotella intermedia (p = 0.017) and Streptococcus aerobic (p = 0.001), Klebsiella aerogens (p = 0.03) and Streptococcus (p = 0.005) and Dialister invisus (p = 0.024) showed statistically significant differences between primary and secondary lesions.
Conclusions
The primary lesions showed significantly higher number of Klebsiella aerogens, Streptococcus, Dialister invisus and secondary lesions with Porphyromonas gingivalis, Prevotella intermedia, Streptococcus respectively.
期刊介绍:
Journal of Oral Biology and Craniofacial Research (JOBCR)is the official journal of the Craniofacial Research Foundation (CRF). The journal aims to provide a common platform for both clinical and translational research and to promote interdisciplinary sciences in craniofacial region. JOBCR publishes content that includes diseases, injuries and defects in the head, neck, face, jaws and the hard and soft tissues of the mouth and jaws and face region; diagnosis and medical management of diseases specific to the orofacial tissues and of oral manifestations of systemic diseases; studies on identifying populations at risk of oral disease or in need of specific care, and comparing regional, environmental, social, and access similarities and differences in dental care between populations; diseases of the mouth and related structures like salivary glands, temporomandibular joints, facial muscles and perioral skin; biomedical engineering, tissue engineering and stem cells. The journal publishes reviews, commentaries, peer-reviewed original research articles, short communication, and case reports.