Optical Control of Membrane Viscosity Modulates ER-to-Golgi Trafficking

IF 10.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Noemi Jiménez-Rojo, , , Suihan Feng, , , Johannes Morstein*, , , Stefanie D. Pritzl, , , Antonino Asaro, , , Sergio López, , , Yun Xu, , , Takeshi Harayama, , , Nynke A. Vepřek, , , Christopher J. Arp, , , Martin Reynders, , , Alexander J. E. Novak, , , Evgeny Kanshin, , , Jan Lipfert, , , Beatrix Ueberheide, , , Manuel Muñiz, , , Theobald Lohmüller, , , Howard Riezman*, , and , Dirk Trauner*, 
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引用次数: 0

Abstract

The lipid composition of cellular membranes is highly dynamic and undergoes continuous remodeling, affecting the biophysical properties critical to biological function. Here, we introduce an optical approach to manipulate membrane viscosity based on an exogenous synthetic fatty acid with an azobenzene photoswitch, termed FAAzo4. Cells rapidly incorporate FAAzo4 into phosphatidylcholine and phosphatidylethanolamine in a concentration- and cell type-dependent manner. This generates photoswitchable PC and PE analogs, which are predominantly located in the endoplasmic reticulum. Irradiation causes a rapid photoisomerization that decreases membrane viscosity with high spatiotemporal precision. We use the resulting “PhotoCells” to study the impact of membrane viscosity on ER-to-Golgi transport and demonstrate that this two-step process has distinct membrane viscosity requirements. Our approach provides an unprecedented way of manipulating membrane biophysical properties directly in living cells and opens novel avenues to probe the effects of viscosity in a wide variety of biological processes.

PhotoCells enable the dynamic control of protein viscosity in living cells. A decrease of membrane viscosity increases the amount of protein recruited at ERES but slows down the transport to Golgi.

膜粘度的光学控制调节er到高尔基体的运输
细胞膜的脂质组成是高度动态的,并经历不断的重塑,影响对生物功能至关重要的生物物理特性。在这里,我们介绍了一种光学方法来操纵膜粘度基于外源合成脂肪酸与偶氮苯光开关,称为FAAzo4。细胞以浓度和细胞类型依赖的方式迅速将FAAzo4结合到磷脂酰胆碱和磷脂酰乙醇胺中。这产生了可光切换的PC和PE类似物,它们主要位于内质网中。辐照引起快速光异构化,以高时空精度降低膜粘度。我们使用得到的“光电池”来研究膜粘度对er到高尔基转运的影响,并证明这两步过程具有不同的膜粘度要求。我们的方法提供了一种前所未有的直接在活细胞中操纵膜生物物理特性的方法,并为探索粘度在各种生物过程中的影响开辟了新的途径。光电池能够动态控制活细胞中的蛋白质粘度。膜黏度的降低增加了ERES募集的蛋白质数量,但减缓了向高尔基体的转运。
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来源期刊
ACS Central Science
ACS Central Science Chemical Engineering-General Chemical Engineering
CiteScore
25.50
自引率
0.50%
发文量
194
审稿时长
10 weeks
期刊介绍: ACS Central Science publishes significant primary reports on research in chemistry and allied fields where chemical approaches are pivotal. As the first fully open-access journal by the American Chemical Society, it covers compelling and important contributions to the broad chemistry and scientific community. "Central science," a term popularized nearly 40 years ago, emphasizes chemistry's central role in connecting physical and life sciences, and fundamental sciences with applied disciplines like medicine and engineering. The journal focuses on exceptional quality articles, addressing advances in fundamental chemistry and interdisciplinary research.
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