Molecular subtypes based on the expression of glutamine metabolism-related genes predict prognosis, tumor microenvironment, and drug therapy response of colon adenocarcinoma.

IF 1.1 4区 医学 Q4 GASTROENTEROLOGY & HEPATOLOGY
Lijian Yu, Xia Lin
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引用次数: 0

Abstract

Background and study aims: Glutamine metabolism (GM) plays an instrumental role in the occurrence and progression of tumors. However, the characteristics of glutamine metabolism-related genes (GMRGs) in colon adenocarcinoma (COAD) have not been elucidated. This project aims to dig out the role of GMRGs in COAD molecular subtypes, prognosis, and tumor microenvironment (TME) to proffer a scientific foundation for the evaluation of COAD prognosis and mechanism research.

Material and methods: Disease data of COAD (including clinical information and gene expression data) and GMRG data were collected from The Cancer Genome Atlas (TCGA)-COAD. GMRGs in COAD were obtained through differential analysis, cluster analysis, protein-protein interaction (PPI) network analysis, and regression analysis for setting up a prognostic model. Disease information was collected from GSE29621 to validate the model. Differences in immunotherapy response and drug sensitivity among high-Riskscore (HR) and low-Riskscore (LR) groups were evaluated.

Results: 45 GMRGs associated with COAD survival rate were identified through univariate analysis. Cluster and survival analyses revealed that these genes could cluster the COAD population into two clusters with great survival differences. A risk model with 10 feature genes was established through regression analysis. Prediction of immunotherapy sensitivity uncovered that the LR group was more sensitive to immunotherapy.

Conclusion: In summary, this project set up a reliable prognostic risk model with GMRGs, which can proffer a feasible basis for the study of prognosis and related mechanisms in COAD.

基于谷氨酰胺代谢相关基因表达的分子亚型预测结肠腺癌的预后、肿瘤微环境和药物治疗反应。
背景与研究目的:谷氨酰胺代谢(Glutamine metabolism, GM)在肿瘤的发生发展中起重要作用。然而,谷氨酰胺代谢相关基因(GMRGs)在结肠腺癌(COAD)中的特征尚未阐明。本项目旨在挖掘GMRGs在COAD分子亚型、预后及肿瘤微环境(tumor microenvironment, TME)中的作用,为COAD预后评价及机制研究提供科学依据。材料与方法:从The Cancer Genome Atlas (TCGA)-COAD收集COAD的疾病数据(包括临床信息和基因表达数据)和GMRG数据。通过差异分析、聚类分析、蛋白-蛋白相互作用(PPI)网络分析和回归分析获得COAD的GMRGs,建立预后模型。从GSE29621中收集疾病信息以验证模型。评估高风险评分组(HR)和低风险评分组(LR)免疫治疗反应和药物敏感性的差异。结果:通过单因素分析,鉴定出45个与COAD生存率相关的GMRGs。聚类和生存分析表明,这些基因可以将COAD群体聚为两个生存差异较大的聚类。通过回归分析,建立了包含10个特征基因的风险模型。免疫治疗敏感性预测显示LR组对免疫治疗更敏感。结论:综上所述,本项目建立了可靠的GMRGs预后风险模型,可为COAD预后及相关机制的研究提供可行性依据。
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来源期刊
Arab Journal of Gastroenterology
Arab Journal of Gastroenterology Medicine-Gastroenterology
CiteScore
2.70
自引率
0.00%
发文量
52
期刊介绍: Arab Journal of Gastroenterology (AJG) publishes different studies related to the digestive system. It aims to be the foremost scientific peer reviewed journal encompassing diverse studies related to the digestive system and its disorders, and serving the Pan-Arab and wider community working on gastrointestinal disorders.
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