Effects of Azithromycin on the Functioning of the Food Web in Freshwater Plankton.

Abstract

High doses of the antibiotic azithromycin in freshwater environments can impact planktonic organisms at both the individual and community levels, influencing interactions at the base of the food web. This study investigated the effects of azithromycin on the natural rotifer community feeding on phytoplankton from a eutrophic water body and its potential impacts on rotifer fitness (impaired mastax movement: slow, irregular or reduced frequency), grazing and mortality following acute exposure. The natural plankton community was exposed to three azithromycin concentrations based on the EC₅₀ value (EC₅₀, 1/2 EC₅₀ and 1/3 EC₅₀) and assessed at different exposure times (24, 48 and 72 h) in the microcosm experiments. The results showed that all azithromycin concentrations reduced the fitness of the rotifers, as indicated by impaired mastax movement and/or slow, irregular or reduced movement frequency. Impairment of mastax movement altered rotifer grazing and the abundance of phytoplankton. The rotifers in the control group suppressed abundant phytoplankton growth, suggesting that azithromycin impairs interspecific interactions between plankton species. Rotifer mortality occurred at 48 h after azithromycin exposure in all treated samples. These findings show that the effects of azithromycin can be observed at different trophic levels, affecting both phytoplankton and zooplankton through altered biotic interactions and suppressed grazing.