Hiromi Miyoshi, Masafumi Otomo, Aki Nishida, Takuto Suzuki, Masashi Yamazaki, Yuki Tani
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引用次数: 0
Abstract
Purpose: The action spectrum for lens epithelial cell (LEC) migration was quantified using an in vitro wound healing assay to provide a basis for preventing posterior capsule opacification (PCO) by optimizing light exposure after cataract surgery.
Methods: This study evaluated the effects of narrowband light in the 400- to 800-nm range with irradiances equal to or lower than those of sunlight (2-10 W/m2) on LEC morphological changes and migration to close a monolayer wound with a 500-µm width.
Results: Under no irradiation (dark) conditions as a negative control, LECs migrated to close the wound in 30 hours. The migration speed in the 400- to 480-nm blue light was lower than that in the dark negative control but was equivalent in the 490- to 540-nm range. The degree of speed reduction was greater for shorter wavelengths and higher irradiances. At wavelengths ≤ 430 nm, cells shrank into dendritic or rounded shapes, with marker staining confirming both as apoptotic. At 570 to 800 nm, the speed was higher in the first 15 hours than that in the dark, after which it decreased.
Conclusions: To the best of our knowledge, this is the first reported action spectrum based on a systematic analysis of LEC migration. Visible light from the blue to far-red region either suppressed or enhanced the LEC migration speed depending on the wavelength and irradiance; that is, including both components potentially decreases and increases PCO risk.
Translational relevance: Our findings can guide light management strategies by optimizing harmful and beneficial light exposure to minimize PCO risk.
期刊介绍：
Translational Vision Science & Technology (TVST), an official journal of the Association for Research in Vision and Ophthalmology (ARVO), an international organization whose purpose is to advance research worldwide into understanding the visual system and preventing, treating and curing its disorders, is an online, open access, peer-reviewed journal emphasizing multidisciplinary research that bridges the gap between basic research and clinical care. A highly qualified and diverse group of Associate Editors and Editorial Board Members is led by Editor-in-Chief Marco Zarbin, MD, PhD, FARVO.
The journal covers a broad spectrum of work, including but not limited to:
Applications of stem cell technology for regenerative medicine,
Development of new animal models of human diseases,
Tissue bioengineering,
Chemical engineering to improve virus-based gene delivery,
Nanotechnology for drug delivery,
Design and synthesis of artificial extracellular matrices,
Development of a true microsurgical operating environment,
Refining data analysis algorithms to improve in vivo imaging technology,
Results of Phase 1 clinical trials,
Reverse translational ("bedside to bench") research.
TVST seeks manuscripts from scientists and clinicians with diverse backgrounds ranging from basic chemistry to ophthalmic surgery that will advance or change the way we understand and/or treat vision-threatening diseases. TVST encourages the use of color, multimedia, hyperlinks, program code and other digital enhancements.
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