A rapid in-lab toolbox for the discrimination of Vibrio natriegens and Escherichia coli cultures

IF 1.9 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of microbiological methods Pub Date : 2025-09-19 DOI:10.1016/j.mimet.2025.107273

Lara S. Möller , José M.M. Santos , A. Rita Silva-Santos , Duarte M.F. Prazeres

引用次数: 0

Abstract

We present a practical toolbox for identifying Vibrio natriegens in laboratory cultures, based on observable phenotypes and species-specific PCR. This workflow enables fast discrimination of V. natriegens from Escherichia coli K-12 and derivatives and microbial contaminants without sequencing or specialized equipment.

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一种快速鉴别产弧菌和大肠杆菌培养物的实验室工具箱。

我们提出了一个实用的工具箱，用于在实验室培养中识别营养弧菌，基于可观察的表型和物种特异性PCR。该工作流程可以快速区分大肠杆菌K-12及其衍生物和微生物污染物，而无需测序或专门的设备。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of microbiological methods 生物-生化研究方法

CiteScore

4.30

自引率

4.50%

发文量

151

审稿时长

29 days

期刊介绍： The Journal of Microbiological Methods publishes scholarly and original articles, notes and review articles. These articles must include novel and/or state-of-the-art methods, or significant improvements to existing methods. Novel and innovative applications of current methods that are validated and useful will also be published. JMM strives for scholarship, innovation and excellence. This demands scientific rigour, the best available methods and technologies, correctly replicated experiments/tests, the inclusion of proper controls, calibrations, and the correct statistical analysis. The presentation of the data must support the interpretation of the method/approach. All aspects of microbiology are covered, except virology. These include agricultural microbiology, applied and environmental microbiology, bioassays, bioinformatics, biotechnology, biochemical microbiology, clinical microbiology, diagnostics, food monitoring and quality control microbiology, microbial genetics and genomics, geomicrobiology, microbiome methods regardless of habitat, high through-put sequencing methods and analysis, microbial pathogenesis and host responses, metabolomics, metagenomics, metaproteomics, microbial ecology and diversity, microbial physiology, microbial ultra-structure, microscopic and imaging methods, molecular microbiology, mycology, novel mathematical microbiology and modelling, parasitology, plant-microbe interactions, protein markers/profiles, proteomics, pyrosequencing, public health microbiology, radioisotopes applied to microbiology, robotics applied to microbiological methods,rumen microbiology, microbiological methods for space missions and extreme environments, sampling methods and samplers, soil and sediment microbiology, transcriptomics, veterinary microbiology, sero-diagnostics and typing/identification.