Zebrafish Unga Is Required for Genomic Maintenance upon Genotoxic Stress and Male Fertility.

IF 2.5 Q3 DEVELOPMENTAL BIOLOGY
Latifa Kazzazy, Flóra Huba, Bálint Lóránt Hausz, Dávid Mező, Viktória Perey-Simon, Bálint Jezsó, Abdulrahman Seddik, Zoran Marinović, Judit Tóth, Angéla Békési, Beáta G Vértessy, Máté Varga
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引用次数: 0

Abstract

DNA repair is a multifaceted biological process that involves multiple pathways to counter the types of damage the genome encounters throughout life. In the past decade zebrafish became a popular model organism to study various aspects of vertebrate DNA repair, and the characterization of several mutant lines deficient in key players of the repair pathways has significantly contributed to our understanding of the roles the corresponding proteins play in the maintenance of genomic integrity. Interestingly, the base-excision repair (BER) pathway remained one of the less characterized DNA repair processes in fish. Here we provide a detailed characterization of zebrafish deficient in one of the key components of BER, the uracil-DNA glycosylase Unga. We show that while these fish are viable, they display an altered response to genotoxic stress and unga mutant males show an interesting form of subfertility.

斑马鱼Unga基因在基因毒性应激和雄性生育能力下的基因组维持是必需的。
DNA修复是一个多方面的生物学过程,涉及多种途径来对抗基因组在生命中遇到的各种损伤。在过去的十年中,斑马鱼成为研究脊椎动物DNA修复各个方面的流行模式生物,并且对修复途径关键参与者缺乏的几个突变系的描述显著有助于我们理解相应蛋白质在维持基因组完整性中的作用。有趣的是,碱基切除修复(BER)途径仍然是鱼类中特征较少的DNA修复过程之一。在这里,我们提供了斑马鱼缺乏BER的关键成分之一的详细特征,尿嘧啶- dna糖基酶Unga。我们发现,虽然这些鱼是可存活的,但它们对基因毒性应激的反应发生了改变,而unga突变的雄性表现出一种有趣的低生育能力。
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来源期刊
Journal of Developmental Biology
Journal of Developmental Biology Biochemistry, Genetics and Molecular Biology-Developmental Biology
CiteScore
4.10
自引率
18.50%
发文量
44
审稿时长
11 weeks
期刊介绍: The Journal of Developmental Biology (ISSN 2221-3759) is an international, peer-reviewed, quick-refereeing, open access journal, which publishes reviews, research papers and communications on the development of multicellular organisms at the molecule, cell, tissue, organ and whole organism levels. Our aim is to encourage researchers to effortlessly publish their new findings or concepts rapidly in an open access medium, overseen by their peers. There is no restriction on the length of the papers; the full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material. Journal of Developmental Biology focuses on: -Development mechanisms and genetics -Cell differentiation -Embryonal development -Tissue/organism growth -Metamorphosis and regeneration of the organisms. It involves many biological fields, such as Molecular biology, Genetics, Physiology, Cell biology, Anatomy, Embryology, Cancer research, Neurobiology, Immunology, Ecology, Evolutionary biology.
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