Qipengyuania marincola sp. nov., Qipengyuania flavescens sp. nov. and Pseudoblastomonas aquimaris sp. nov., three novel members of the family Erythrobacteraceae isolated from seawater.

IF 2 3区生物学 Q4 MICROBIOLOGY

International journal of systematic and evolutionary microbiology Pub Date : 2025-09-01 DOI:10.1099/ijsem.0.006924

Tian-Pu Li, Dao-Feng Zhang, Zi-Yue Fu, Meng-Han Huang

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Abstract

Three Gram-stain-negative, aerobic, rod-shaped, non-motile and orange-pigmented bacteria, designated as XHP0207^T, XHP0211^T and WL0013^T, were isolated from surface seawater collected from Nantong City, the Yellow Sea of China. Cells were positive for catalase and oxidase. These strains were all optimally grown at 28 ℃, pH 7.0 and 3% (w/v) NaCl and possessed Q-10 as the respiratory quinone. The 16S rRNA gene sequence analysis indicated that strains XHP0207^T, XHP0211^T and WL0013^T showed the highest sequence similarity to Qipengyuania profundimaris G39^T (99.0%), Q. profundimaris G39^T (100%) and Alteriqipengyuania flavescens SCSIO 75105^T (100%), respectively. However, a phylogenetic tree based on the 288 orthologous clusters indicated that both XHP0207^T and XHP0211^T were closely related to Qipengyuania proteolytica 6B39^T, and WL0013^T was closely related to Alterqipengyuania flavescens SCSIO 75105^T. The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values among strains XHP0207^T, XHP0211^T and WL0013^T and closely related type strains were all below the species delineation cutoffs of 95%, 95% and 70%, respectively, though the values exceeded between strain XHP0207^T and a non-type strain. The genomic DNA G+C contents of strains XHP0207^T, XHP0211^T and WL0013^T were 63.0%, 63.0% and 65.5%, respectively. Based on the phenotypic, phylogenetic and chemotaxonomic characterizations, strains XHP0207^T and XHP0211^T represent two novel Qipengyuania species, and we thus propose the names Qipengyuania marincola sp. nov. (type strain, XHP0207^T=MCCC 1K07527^T=GDMCC 1.3090^T=JCM 35571^T) and Qipengyuania flavescens sp. nov. (type strain, XHP0211^T=MCCC 1K07528^T=GDMCC 1.3091^T=JCM 35572^T). Strain WL0013^T represents a novel Pseudoblastomonas species, for which the name Pseudoblastomonas aquimaris sp. nov. (type strain, WL0013^T=JCM 34654^T=GDMCC1.2439^T) is proposed. In addition, Alterqipengyuania flavescens is reclassified as Pseudoblastomonas flavescens comb. nov. (type strain, KCTC 92502^T=MCCC 1K07993^T=SCSIO 75105^T).

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从海水中分离到的红杆菌科新成员marincola、flavescens和Pseudoblastomonas aquimaris。

从黄海南通市表层海水中分离到革兰氏阴性、好氧、棒状、不活动、橙色色素的3株细菌，编号为XHP0207T、XHP0211T和WL0013T。细胞过氧化氢酶和氧化酶阳性。这些菌株均在28℃、pH 7.0和3% (w/v) NaCl条件下生长，其呼吸醌含量为Q-10。16S rRNA基因序列分析表明，菌株XHP0207T、XHP0211T和WL0013T分别与Qipengyuania profunddimaris G39T（99.0%）、Q. profunddimaris G39T（100%）和alteripipengyuania flavescens SCSIO 75105T（100%）序列相似性最高。而基于288个同源聚类的系统进化树分析结果显示，XHP0207T和XHP0211T与齐彭元ania proteolytica 6B39T亲缘关系较近，WL0013T与Alterqipengyuania flavescens SCSIO 75105T亲缘关系较近。菌株XHP0207T、XHP0211T和WL0013T与近缘型菌株的平均核苷酸同源性、平均氨基酸同源性和数字DNA-DNA杂交值分别低于95%、95%和70%的种划分截断值，但超过了菌株XHP0207T与非型菌株的划分截断值。菌株XHP0207T、XHP0211T和WL0013T基因组DNA G+C含量分别为63.0%、63.0%和65.5%。基于表型、系统发育和化学分类特征，本文提出了两种新型齐蓬原属植物XHP0207T和XHP0211T的命名，分别为齐蓬原属植物marincola sp. 11（型菌株XHP0207T=MCCC 1K07527T=GDMCC 1.3090T=JCM 35571T）和flavescens sp. 11（型菌株XHP0211T=MCCC 1K07528T=GDMCC 1.3091T=JCM 35572T）。菌株WL0013T是一种新的假母细胞单胞菌，建议将其命名为Pseudoblastomonas aquimaris sp. nov（型菌株，WL0013T=JCM 34654T=GDMCC1.2439T）。另外，将Alterqipengyuania flavescens重新分类为Pseudoblastomonas flavescens comb。11 .（型应变，KCTC 92502T=MCCC 1K07993T=SCSIO 75105T）。

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来源期刊

International journal of systematic and evolutionary microbiology 生物-微生物学

CiteScore

5.20

自引率

21.40%

发文量

426

审稿时长

1 months

期刊介绍： Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names. The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including: Identification, characterisation and culture preservation Microbial evolution and biodiversity Molecular environmental work with strong taxonomic or evolutionary content Nomenclature Taxonomy and phylogenetics.