Genome-wide identification of bHLH gene family and screening of genes related to prickle development in Zanthoxylum armatum.

IF 2.7 4区生物学 Q2 PLANT SCIENCES

Functional Plant Biology Pub Date : 2025-07-01 DOI:10.1071/FP24345

Yuwei Yi, Hualin Zou, Nuo Wang, Yansheng Xue, Weiwei Zhang, Feng Xu, Jiabao Ye, Yongling Liao

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引用次数: 0

Abstract

Zanthoxylum armatum has edible and medicinal value but its prickles make harvesting difficult. The bHLH gene family is vital in regulating physiological and developmental processes. One hundred and ninety-five ZabHLH genes from its genome were grouped into 11 subgroups and 23 subfamilies. Members of the bHLH IIIf subfamily play an important role in trichome development, and ZabHLH22 , ZabHLH110 , ZabHLH161 , and ZabHLH194 , which belong to this subfamily, were selected as candidate genes. Chromosomal localization analysis showed that 165 of 195 ZabHLHs were unevenly distributed on 31 chromosomes, and 30 ZabHLHs were localized to unanchored scaffolds. The expansion of ZabHLHs mainly includes dispersed replication and whole-genome duplication or segmental replication. Fourty-seven cis -acting elements were predicted in the promoters of ZabHLHs , with hormone-responsive elements being the most abundant. Expression profiles of four candidate genes were analyzed in two Z. armatum cultivars. Trichome development is regulated by hormones such as methyl jasmonate, salicylic acid, and auxin. The qRT-PCR results indicate that four candidate genes respond to the stress induced by these three hormones. We predict that ZabHLH110 , ZabHLH161 , and ZabHLH194 are most likely involved in prickle development. The results are helpful to further explore the potential roles and mechanisms of ZabHLHs in the development of Z. armatum prickles.

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花椒bHLH基因家族的全基因组鉴定及刺发育相关基因的筛选。

Zanthoxylum armatum具有食用和药用价值，但其刺使收获困难。bHLH基因家族在调节生理和发育过程中至关重要。从其基因组中提取的195个ZabHLH基因被划分为11个亚群和23个亚家族。bHLH IIIf亚家族的成员在毛状体发育中起重要作用，我们选择了属于该亚家族的ZabHLH22、ZabHLH110、ZabHLH161和ZabHLH194作为候选基因。染色体定位分析显示，195个ZabHLHs中的165个不均匀分布在31条染色体上，30个定位在未锚定的支架上。ZabHLHs的扩增主要包括分散复制和全基因组复制或片段复制。在ZabHLHs的启动子中预测了47个顺式作用元件，其中激素响应元件最为丰富。分析了4个候选基因在2个柽柳品种中的表达谱。毛状体的发育受茉莉酸甲酯、水杨酸和生长素等激素的调节。qRT-PCR结果表明，4个候选基因对这3种激素诱导的应激有应答。我们预测ZabHLH110、ZabHLH161和ZabHLH194最有可能参与刺痛的发育。这一结果有助于进一步探讨ZabHLHs在番荔枝皮发育中的潜在作用及其机制。

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来源期刊

Functional Plant Biology 生物-植物科学

CiteScore

5.50

自引率

3.30%

发文量

156

审稿时长

1 months

期刊介绍： Functional Plant Biology (formerly known as Australian Journal of Plant Physiology) publishes papers of a broad interest that advance our knowledge on mechanisms by which plants operate and interact with environment. Of specific interest are mechanisms and signal transduction pathways by which plants adapt to extreme environmental conditions such as high and low temperatures, drought, flooding, salinity, pathogens, and other major abiotic and biotic stress factors. FPB also encourages papers on emerging concepts and new tools in plant biology, and studies on the following functional areas encompassing work from the molecular through whole plant to community scale. FPB does not publish merely phenomenological observations or findings of merely applied significance. Functional Plant Biology is published with the endorsement of the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and the Australian Academy of Science. Functional Plant Biology is published in affiliation with the Federation of European Societies of Plant Biology and in Australia, is associated with the Australian Society of Plant Scientists and the New Zealand Society of Plant Biologists.