Modulation of ion transporter genes of salt-stressed sorghum (Sorghum bicolor L. Moench) by foliar application of digitoxin.

IF 2.7 4区生物学 Q2 PLANT SCIENCES

Functional Plant Biology Pub Date : 2025-09-01 DOI:10.1071/FP25031

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引用次数: 0

Abstract

Salinity poses a major threat to cereal crops such as sorghum. The foliar application of digitoxin at concentrations of 50, 100, and 200ppm was tested for its potential to alleviate salt stress in sorghum (Sorghum bicolor ) exposed to 200mM NaCl. Various growth parameters were analyzed, such as relative water content, malondialdehyde (MDA), osmoregulatory compunds (soluble carbohydrates and proline), ionic markers (Na+ and K+ levels in shoots and roots), and the expression of specific ion transporter genes including NHX , SOS1 , AKT1 , PPV , and PHA1 during the seedling stage. Digitoxin treatment significantly enhanced biochemical and ionic characteristics in salt-stressed plants by enhancing the membrane stability index and reducing MDA levels while boosting soluble carbohydrates, free amino acids, and proline. Real-time PCR showed that digitoxin application triggered the upregulation of genes promoting Na+ and K+ balance and reducing ion toxicity. This study underscores the potential role of digitoxin in improving salt tolerance through its influence on the regulation of ion transporter gene expression specific for K+ and Na+ ion transport and homeostasis. The effect of digitoxin on the ion transporters seems to be dose-dependent. The mechanism of digitoxin's effect on ion transporter gene expression of salt-stressed plants is discussed.

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洋地黄毒素对盐胁迫高粱离子转运体基因的调控

盐碱化对高粱等谷类作物构成重大威胁。研究了在200mM NaCl胁迫下，叶面施用50ppm、100ppm和200ppm洋地黄毒素对高粱（高粱双色）盐胁迫的缓解作用。分析了幼苗期相对含水量、丙二醛（MDA）、渗透调节化合物（可溶性碳水化合物和脯氨酸）、离子标记物（茎和根中Na+和K+水平）以及特定离子转运基因NHX、SOS1、AKT1、PPV和PHA1的表达等生长参数。地地黄毒素处理通过提高膜稳定性指数、降低丙二醛水平、提高可溶性碳水化合物、游离氨基酸和脯氨酸水平，显著改善了盐胁迫植物的生化和离子特性。实时荧光定量PCR结果显示，地地黄毒素可引发促进Na+和K+平衡、降低离子毒性的基因上调。本研究强调了洋地黄毒素通过影响K+和Na+离子运输和体内平衡的离子转运体基因表达来提高盐耐受性的潜在作用。洋地黄素对离子转运体的影响似乎是剂量依赖性的。探讨了洋地黄毒素影响盐胁迫植物离子转运体基因表达的机制。

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来源期刊

Functional Plant Biology 生物-植物科学

CiteScore

5.50

自引率

3.30%

发文量

156

审稿时长

1 months

期刊介绍： Functional Plant Biology (formerly known as Australian Journal of Plant Physiology) publishes papers of a broad interest that advance our knowledge on mechanisms by which plants operate and interact with environment. Of specific interest are mechanisms and signal transduction pathways by which plants adapt to extreme environmental conditions such as high and low temperatures, drought, flooding, salinity, pathogens, and other major abiotic and biotic stress factors. FPB also encourages papers on emerging concepts and new tools in plant biology, and studies on the following functional areas encompassing work from the molecular through whole plant to community scale. FPB does not publish merely phenomenological observations or findings of merely applied significance. Functional Plant Biology is published with the endorsement of the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and the Australian Academy of Science. Functional Plant Biology is published in affiliation with the Federation of European Societies of Plant Biology and in Australia, is associated with the Australian Society of Plant Scientists and the New Zealand Society of Plant Biologists.