Development of Electrochemical Sensor Modified with Murumuru Biochar for Nicotinic Acid Determination

IF 2.3 3区化学 Q2 CHEMISTRY, ANALYTICAL

Electroanalysis Pub Date : 2025-09-22 DOI:10.1002/elan.70061

Gabriela Françozo Vilela, Juliana Garcia, Matheus Julien Ferreira Bazzana, Pedro Augusto dos Santos, Zuy Maria Magriotis, Sérgio Scherrer Thomasi, Leonardo Luiz Okumura, Tatiana Cardoso e Bufalo, Adelir Aparecida Saczk

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Abstract

Nicotinic acid (NA) is a free form of vitamin B3, and its altered levels in the human body can lead to serious clinical complications. Therefore, analytical methods to monitor this molecule must be developed. Voltammetric techniques have become viable because of their short analysis time and low associated cost. Thus, in this study, murumuru biochar (MBC) electrode was evaluated for NA determination using the differential pulse voltammetry (DPV) technique. The proportion of carbon paste was adjusted through the design of mixtures, resulting in proportions of 36.6% binder, 31.7% MBC, and 31.7% graphite. KCl (0.1 mol L⁻¹) acidified with HClO₄ at pH 2 was selected as the supporting electrolyte. DPV analyses were performed with a step of 5 mV, a pulse amplitude of 100 mV, a time interval of 75 ms, and a modulation time of 2 ms. The analytical curve presented an r² of 0.999 in a linear range from 4 to 100 μmol L⁻¹. The limit of detection (LOD) was 0.36 μmol L⁻¹, and the limit of quantification (LOQ) was 1.20 μmol L⁻¹. Based on the obtained analytical curve, NA was quantified in samples of multivitamins and synthetic urine, and satisfactory results were shown.

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木质素生物炭改性电化学传感器测定烟酸的研制

烟酸（NA）是维生素B3的游离形式，它在人体内的水平改变会导致严重的临床并发症。因此，必须开发监测该分子的分析方法。伏安技术因其分析时间短、成本低而变得可行。因此，在本研究中，采用差分脉冲伏安法（DPV）技术评估了murumuru生物炭（MBC）电极测定NA的可行性。通过配合比的设计，调整碳浆的掺量，粘结剂用量为36.6%，MBC用量为31.7%，石墨用量为31.7%。选择HClO4在pH 2下酸化的KCl （0.1 mol L−1）作为支撑电解质。DPV分析的步进为5 mV，脉冲幅度为100 mV，时间间隔为75 ms，调制时间为2 ms。在4 ~ 100 μmol L−1的线性范围内，曲线r2为0.999。检测限为0.36 μmol L−1，定量限为1.20 μmol L−1。根据所得到的分析曲线，对复合维生素和合成尿液中的NA进行了定量分析，结果令人满意。

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来源期刊

Electroanalysis 化学-电化学

CiteScore

6.00

自引率

3.30%

发文量

222

审稿时长

2.4 months

期刊介绍： Electroanalysis is an international, peer-reviewed journal covering all branches of electroanalytical chemistry, including both fundamental and application papers as well as reviews dealing with new electrochemical sensors and biosensors, nanobioelectronics devices, analytical voltammetry, potentiometry, new electrochemical detection schemes based on novel nanomaterials, fuel cells and biofuel cells, and important practical applications. Serving as a vital communication link between the research labs and the field, Electroanalysis helps you to quickly adapt the latest innovations into practical clinical, environmental, food analysis, industrial and energy-related applications. Electroanalysis provides the most comprehensive coverage of the field and is the number one source for information on electroanalytical chemistry, electrochemical sensors and biosensors and fuel/biofuel cells.