Scaffolds based on organosilane-functionalized cellulose microfibrillated cryogels for 3D cell culture in extracellular matrix

IF 4.8 2区 工程技术 Q1 MATERIALS SCIENCE, PAPER & WOOD
Lara Vasconcellos Ponsoni, Marina Kauling de Almeida, Beatriz Tomé, Natasha Maurmann, Anna Luíza Kern, Patricia Pranke, Sabrina Arcaro, Beatriz Merillas Valero, Matheus Vinicius Gregory Zimmermann
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Abstract

Three-dimensional porous scaffolds play an important role in tissue support and growth and are widely used in cell culture within the extracellular matrix (ECM). This approach represents a significant advancement in the biomedical field, enabling the replacement of traditional two-dimensional (2D) cell cultures and reducing the need for animal testing, which presents physiological, metabolic, and high-cost limitations. Among the promising materials for scaffold development, cellulose stands out as a renewable and biocompatible biopolymer whose structural properties can be adjusted through different drying processes and chemical modifications. Despite advances in the use of cellulose aerogels and cryogels as biomaterials, the influence of different organosilanes in the functionalization of these structures remains underexplored, particularly regarding cell adhesion and proliferation. In this context, this study aimed to develop and characterize scaffolds based on microfibrillated cellulose cryogels obtained through freeze-drying and functionalized via vapor deposition with different organosilanes: tetraethoxysilane (TEOS), triethoxyvinylsilane (TEVS), 3-aminopropyltriethoxysilane (APTES), and 3-glycidyloxypropyltrimethoxysilane (GPTMS). The cryogels were characterized in terms of their morphology, chemical properties and cytotoxicity. Cryogels treated with TEOS and GPTMS demonstrated better adhesion and cell viability in assays with MRC-5 fibroblasts and PC-12 neural cells, making them promising candidates for 3D cell culture applications. The results of this study demonstrate that functionalizing cellulose cryogels enhances cell adhesion and proliferation, establishing these materials as potential scaffolds for cell culture and tissue engineering. These findings contribute to advancing the replacement of 2D models and in vivo assays, promoting the development of more effective biomimetic systems for biomedical applications.

Abstract Image

基于有机硅烷功能化纤维素微纤化低温支架的细胞外基质三维细胞培养
三维多孔支架在组织支撑和生长中起着重要的作用,在细胞外基质(ECM)内的细胞培养中得到了广泛的应用。这种方法代表了生物医学领域的重大进步,取代了传统的二维(2D)细胞培养,减少了对动物试验的需求,这带来了生理、代谢和高成本的限制。纤维素是一种可再生的、具有生物相容性的生物聚合物,其结构特性可以通过不同的干燥工艺和化学修饰来调节。尽管在使用纤维素气凝胶和低温凝胶作为生物材料方面取得了进展,但不同有机硅烷对这些结构功能化的影响仍未得到充分探索,特别是对细胞粘附和增殖的影响。在此背景下,本研究旨在开发和表征微纤化纤维素低温支架,该支架通过冷冻干燥获得,并通过气相沉积功能化不同的有机硅烷:四乙氧基硅烷(TEOS),三乙氧基乙烯基硅烷(TEVS), 3-氨基丙基三乙氧基硅烷(APTES)和3-缩水氧基丙基三甲氧基硅烷(GPTMS)。从形态、化学性质和细胞毒性等方面对其进行了表征。在与MRC-5成纤维细胞和PC-12神经细胞的实验中,TEOS和GPTMS处理的冷冻细胞表现出更好的粘附性和细胞活力,使其成为3D细胞培养应用的有希望的候选者。本研究的结果表明,功能化的纤维素冷冻材料可以增强细胞的粘附和增殖,使这些材料成为细胞培养和组织工程的潜在支架材料。这些发现有助于推动二维模型和体内实验的替代,促进更有效的生物医学应用仿生系统的发展。
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来源期刊
Cellulose
Cellulose 工程技术-材料科学:纺织
CiteScore
10.10
自引率
10.50%
发文量
580
审稿时长
3-8 weeks
期刊介绍: Cellulose is an international journal devoted to the dissemination of research and scientific and technological progress in the field of cellulose and related naturally occurring polymers. The journal is concerned with the pure and applied science of cellulose and related materials, and also with the development of relevant new technologies. This includes the chemistry, biochemistry, physics and materials science of cellulose and its sources, including wood and other biomass resources, and their derivatives. Coverage extends to the conversion of these polymers and resources into manufactured goods, such as pulp, paper, textiles, and manufactured as well natural fibers, and to the chemistry of materials used in their processing. Cellulose publishes review articles, research papers, and technical notes.
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