Role of skeletal muscle vasodilatory pathway in adaptive thermogenesis in Sunit Mongolian sheep

IF 2.9 2区 生物学 Q2 BIOLOGY
F.H. Meng , L. Wang , T. Zhou , L.W. Chang , Y.Y. Zi , R.N. Su , M. Zhao , X.Y. Zhou , D. Zhang , W.G. Zhang , C.X. Liu
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引用次数: 0

Abstract

The Sunite Mongolian sheep is one of the superior livestock breeds in Inner Mongolia, capable of surviving and reproducing on the Xilingol Grassland at temperatures below −30 °C, demonstrating remarkable cold adaptation. However, research on adaptive thermogenesis in its muscle tissue remains limited. To investigate skeletal muscle's role in adaptive thermogenesis, shoulder muscle tissues were collected from three healthy castrated male sheep each in winter (January) and summer (July) for RNA sequencing (RNA-seq) and differential expression analysis. Differentially expressed genes (DEGs) were primarily enriched in pathways associated with starvation response, hormonal regulation, and energy metabolism. Gene expression analysis revealed significant upregulation of nitric oxide synthase 3 (NOS3) and adrenergic receptor-β2 (ADRB2) in the adrenergic-cyclic guanosine monophosphate (cGMP)- protein kinase G(PKG)vasodilatory signaling pathway during winter (P < 0.01). Further validation showed that ADRB2 was extensively expressed in skeletal muscle vascular endothelial cells, with significantly increased winter expression. Subsequent in vivo experiments confirmed these results, intravenous injection of isoproterenol (ISO) dose-dependently increased cGMP concentration and NOS3 expression (P < 0.05). In summary, we speculate that epinephrine binds to ADRB2 in the skeletal muscle of Sunite Mongolian sheep during winter, activating intracellular NOS3 to synthesize nitric oxide (NO). Through the cGMP-PKG signaling pathway, this induces vasodilation, thereby transporting more nutrients and enhancing metabolic energy production and heat generation, primarily via glucose metabolism, to adapt to cold environments. This study provides new insights into cold adaptation in sheep, suggesting ADRB2/NOS3 as potential molecular markers for breeding cold-resistant breeds to enhance farming efficiency.
苏尼特蒙古羊骨骼肌血管扩张途径在适应性产热中的作用
苏尼特蒙古羊是内蒙古优良的家畜品种之一,能够在零下30℃的锡林郭勒草原上生存和繁殖,具有较强的冷适应能力。然而,对其肌肉组织的适应性产热的研究仍然有限。为了研究骨骼肌在适应性产热中的作用,在冬季(1月)和夏季(7月)分别采集3只健康去势公羊的肩部肌肉组织,进行RNA测序(RNA-seq)和差异表达分析。差异表达基因(DEGs)主要富集于与饥饿反应、激素调节和能量代谢相关的途径中。基因表达分析显示,冬季期间一氧化氮合酶3 (NOS3)和肾上腺素能受体-β2 (ADRB2)在肾上腺素能-环鸟苷单磷酸(cGMP)-蛋白激酶G(PKG)血管舒张信号通路中的表达显著上调(P < 0.01)。进一步验证表明,ADRB2在骨骼肌血管内皮细胞中广泛表达,且冬季表达量显著增加。随后的体内实验证实了这些结果,静脉注射异丙肾上腺素(ISO)剂量依赖性地增加了cGMP浓度和NOS3表达(P < 0.05)。综上所述,我们推测肾上腺素在冬季与苏尼特蒙古羊骨骼肌中的ADRB2结合,激活细胞内NOS3合成一氧化氮(NO)。这通过cGMP-PKG信号通路诱导血管舒张,从而运输更多的营养物质,增强代谢能量的产生和热的产生,主要通过葡萄糖代谢来适应寒冷环境。本研究为研究绵羊的冷适应提供了新的思路,提示ADRB2/NOS3可作为选育抗寒品种以提高养殖效率的潜在分子标记。
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来源期刊
Journal of thermal biology
Journal of thermal biology 生物-动物学
CiteScore
5.30
自引率
7.40%
发文量
196
审稿时长
14.5 weeks
期刊介绍: The Journal of Thermal Biology publishes articles that advance our knowledge on the ways and mechanisms through which temperature affects man and animals. This includes studies of their responses to these effects and on the ecological consequences. Directly relevant to this theme are: • The mechanisms of thermal limitation, heat and cold injury, and the resistance of organisms to extremes of temperature • The mechanisms involved in acclimation, acclimatization and evolutionary adaptation to temperature • Mechanisms underlying the patterns of hibernation, torpor, dormancy, aestivation and diapause • Effects of temperature on reproduction and development, growth, ageing and life-span • Studies on modelling heat transfer between organisms and their environment • The contributions of temperature to effects of climate change on animal species and man • Studies of conservation biology and physiology related to temperature • Behavioural and physiological regulation of body temperature including its pathophysiology and fever • Medical applications of hypo- and hyperthermia Article types: • Original articles • Review articles
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