Protective outcomes of Zingiber officinale leaves ethanol extract on ovarian oxidative stress and hormonal functions in malaria mice

Food chemistry advances Pub Date : 2025-09-08 DOI:10.1016/j.focha.2025.101110

Joel Okpoghono , Joy Onyewonuwa Acha , Solomon Ugochukwu Okom , Cyril Chuckwudi Dunkwu , Innocent Onyesom

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Abstract

This study aimed to evaluate the protective outcomes of ethanol extract of Zingiber officinale (EEZo) leaves on ovarian oxidative stress and hormonal functions in malaria mice. Mice were grouped into 6 groups of 6 mice each. After mice were euthanized, serum and ovarian tissues were taken for biochemical analyses. Parameters such as malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), catalase (CAT), and nitric oxide (NO) were assessed in the ovary. Estradiol, Anti-Mullerian Hormone (AMH), Luteinizing Hormone (LH), and Follicle Stimulating Hormone (FSH) in the serum were estimated using the ELISA Kit. The results showed significant low value of MDA (21.45 ± 0.71 μg/g wet tissue), high SOD (0.30 ± 0.14 μg/g wet tissue) and GSH (11.50 ± 1.27 μmol /g wet tissue) after treatment with EEZo at 400 mg/kg/d in comparison with malaria infected mice (60.0 ± 0.11 μg/g wet tissue; 0.05 ± 0.02 μg/g wet tissue; 1.50 ± 0.85 μmol /g wet tissue). Also, significant increase were observed in LH, FSH, AMH and estradiol in malaria infected group treated with EEZo and standard drug (Artemether and Lumefantrine (lonart). In conclusion, it is suggested that EEZo and Lonart could reduce oxidative stress and improve ovarian function in malaria mice.

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生姜叶乙醇提取物对疟疾小鼠卵巢氧化应激和激素功能的保护作用

本研究旨在探讨鲜姜叶乙醇提取物对疟疾小鼠卵巢氧化应激和激素功能的保护作用。将小鼠分为6组，每组6只。小鼠安乐死后，取血清和卵巢组织进行生化分析。测定卵巢丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽（GSH）、过氧化氢酶（CAT）、一氧化氮（NO）等指标。采用ELISA试剂盒测定血清中雌二醇、抗苗勒管激素（AMH）、促黄体生成素（LH）和促卵泡激素（FSH）的含量。结果显示，与疟疾感染小鼠（60.0±0.11 μg/g、0.05±0.02 μg/g、1.50±0.85 μmol /g）相比，400 mg/kg/d EEZo处理后MDA（21.45±0.71 μg/g）降低，SOD（0.30±0.14 μg/g）和GSH（11.50±1.27 μmol /g）升高。在疟疾感染组中，EEZo和标准药物（蒿甲醚和氨芳曲明）治疗后，LH、FSH、AMH和雌二醇均显著升高。综上所述，EEZo和Lonart可降低疟疾小鼠氧化应激，改善卵巢功能。

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