Prevalence, molecular characterization, and therapeutic management of Buxtonella sulcata in buffaloes from Western Uttar Pradesh, India

IF 1.4 Q3 PARASITOLOGY

Veterinary parasitology, regional studies and reports Pub Date : 2025-09-10 DOI:10.1016/j.vprsr.2025.101342

Prem Sagar Maurya , Ramakant , Arbind Singh , Vikas Jaiswal , Tarun Kumar Sarkar , Ayush Pal , Surendra Upadhyay , Amit Kumar Verma

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引用次数: 0

Abstract

Buxtonella sulcata is an opportunistic protozoan parasite commonly affecting ruminants, with significant infection rates reported in cattle and buffaloes worldwide. This large cyst-forming ciliate shares morphological similarities with Balantidium coli, a known parasite of humans and pigs. In the present study, 512 fecal samples were collected from diarrheic and non-diarrheic buffaloes across five districts Meerut, Saharanpur, Muzaffarnagar, Baghpat, and Hapur in Western Uttar Pradesh, India, between April 2023 and March 2024. Samples were subjected to both microscopic examination and polymerase chain reaction (PCR) targeting the 18S rRNA gene, followed by sequencing and phylogenetic analysis. The overall prevalence of B. sulcata was found to be 33.59 % by microscopy and 35.16 % by PCR. Confirmed cases were treated with halquinol bolus administered orally and long-acting oxytetracycline administered intramuscularly. This study highlights the notable presence of B. sulcata in the buffalo population of Western Uttar Pradesh and emphasizes the importance of molecular diagnostics for accurate detection. Furthermore, the research supports the efficacy of a combination therapeutic regimen and recommends its strategic application to manage buxtonellosis in affected regions.

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印度北方邦西部水牛中布氏通体的流行、分子特征和治疗管理

沟巴克通体是一种通常影响反刍动物的机会性原生动物寄生虫，据报道，全世界牛和水牛的感染率很高。这种大的囊状纤毛虫与已知的人类和猪的寄生虫大肠杆菌在形态上有相似之处。在本研究中，在2023年4月至2024年3月期间，从印度西部北方邦的密鲁特、萨哈兰普尔、穆扎法纳加尔、巴格帕特和哈普尔五个地区收集了512份腹泻和非腹泻水牛的粪便样本。对样品进行显微镜检查和靶向18S rRNA基因的聚合酶链反应（PCR），然后进行测序和系统发育分析。光镜下和PCR检测结果分别为33.59%和35.16%。确诊病例采用口服氟喹诺丸和肌注长效土霉素治疗。这项研究强调了在西部北方邦水牛种群中显著存在的B. sulcata，并强调了分子诊断对准确检测的重要性。此外，该研究支持一种联合治疗方案的有效性，并建议在受影响地区战略性地应用该方案来管理布氏体病。

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来源期刊

Veterinary parasitology, regional studies and reports Multiple-

CiteScore

2.90

自引率

7.10%

发文量

126

审稿时长

97 days

期刊介绍： Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).