Synaptic pruning following NMDAR-dependent LTD preferentially affects isolated synapses

IF 4.1 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

iScience Pub Date : 2025-08-29 DOI:10.1016/j.isci.2025.113093

Côme Camus , Léa Leval , Viviana Villicana-Munoz , Sarka Jelinkova , Benjamin Compans , Frédéric Gambino , Etienne Herzog , Daniel Choquet , Eric Hosy

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Abstract

Long-term depression (LTD) is an activity-dependent decrease in synaptic strength. This state initiates either a re-potentiation or a loss, aka pruning, of the synapse within hours to days following its induction. However, the precise relationship between LTD and synaptic pruning remains elusive. Using various imaging techniques and electrophysiology on neuronal cell culture, we reveal a direct relationship between LTD and synaptic pruning. All synapses that exhibit NMDAR-dependent LTD are pruned unless counteracted by input-specific activity. Additionally, we identify the post-synaptic ion channels pivotal for sensing pre-synaptic activity. Finally, we find that neighboring synapses displaying coordinated activity act as mutual safeguards against pruning. These distinctive properties mean that LTD-dependent synaptic pruning has a major impact on synapses that do not contribute to responses involved in network activity.

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nmdar依赖性LTD后的突触修剪优先影响孤立突触

长期抑郁（LTD）是一种活动依赖性的突触强度下降。这种状态在诱导后的数小时到数天内启动突触的再增强或损失，即修剪。然而，LTD与突触修剪之间的确切关系仍然难以捉摸。利用各种成像技术和神经细胞培养的电生理学，我们揭示了LTD与突触修剪之间的直接关系。所有表现出nmdar依赖性LTD的突触都被修剪，除非被输入特定活动抵消。此外，我们确定突触后离子通道是感知突触前活动的关键。最后，我们发现显示协调活动的相邻突触作为相互保护防止修剪。这些独特的特性意味着依赖于有限突触的突触修剪对那些不参与网络活动的突触产生重大影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

iScience Multidisciplinary-Multidisciplinary

CiteScore

7.20

自引率

1.70%

发文量

1972

审稿时长

6 weeks

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