SNHG16 promotes breast cancer progression by regulating the hsa-let-7b-5p/AURKA axis

IF 2.2 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemistry and Biophysics Reports Pub Date : 2025-09-18 DOI:10.1016/j.bbrep.2025.102271

Zipeng Qiao , Yongjun Tang , Shengle Li , Qiufeng Lao , Yu Xing , Qingquan Zhang , Songying Pan , Chaoyong Bei , Weiyi Pang , Hui Liu

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引用次数: 0

Abstract

Background

Long non-coding RNAs (lncRNAs) play crucial roles in the progression of breast cancer (BC). The lncRNA small nuclear RNA host gene 16 (SNHG16), represents a lncRNA associated with tumor development. This study focuses on SNHG16 and its regulatory role in BC progression, validated through microarray analysis and in vitro experiments.

Method

The GEO datasets GSE65194 and GSE61304 were used to identify differentially expressed lncRNAs, GSE41922 and GSE45666 for differentially expressed miRNAs, and GSE29431 and GSE42568 for differentially expressed mRNAs. The TCGA database was used to validate the genes identified in the previous analyses. The regulatory pathways of SNHG16 were identified through differential gene analysis, target gene prediction, functional enrichment analysis, and protein-protein interaction network analysis. RT-qPCR, Western blot, CCK-8, cell migration, and cell knockdown experiments were performed for validation.

Results

Our study found that elevated expression levels of SNHG16 were associated with BC cell proliferation and poor prognosis. According to an in vitro knockdown assay, SNHG16 silencing inhibits the progression of BC. SNHG16 was found to positively regulate AURKA levels as a competitive sponge for hsa-let-7b-5p.

Conclusion

The activity of the lncRNA SNHG16 can be triggered via the hsa-let-7b-5p/AURKA axes. These findings shed light on the novel molecular mechanisms underlying BC progression.

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SNHG16通过调节hsa-let-7b-5p/AURKA轴促进乳腺癌进展

长链非编码rna （lncRNAs）在乳腺癌（BC）的进展中起着至关重要的作用。lncRNA小核RNA宿主基因16 （SNHG16）是一种与肿瘤发生相关的lncRNA。本研究的重点是SNHG16及其在BC进展中的调节作用，并通过微阵列分析和体外实验验证。方法使用GEO数据集GSE65194和GSE61304鉴定差异表达的lncrna， GSE41922和GSE45666鉴定差异表达的mirna， GSE29431和GSE42568鉴定差异表达的mrna。使用TCGA数据库验证先前分析中鉴定的基因。通过差异基因分析、靶基因预测、功能富集分析和蛋白-蛋白互作网络分析，确定了SNHG16的调控途径。通过RT-qPCR、Western blot、CCK-8、细胞迁移和细胞敲除实验进行验证。结果本研究发现SNHG16表达水平升高与BC细胞增殖和预后不良相关。根据体外敲除实验，SNHG16沉默抑制BC的进展。SNHG16作为hsa-let-7b-5p的竞争性海绵，正调节AURKA水平。结论lncRNA SNHG16的活性可通过hsa-let-7b-5p/AURKA轴触发。这些发现揭示了BC进展的新分子机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

191

审稿时长

59 days

期刊介绍： Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.