Ferrocene-functionalized ZIF-8@Pt nanoparticles-engineered dual-mode aptasensor: Ratiometric electrochemical/impedimetric detection of chlorpyrifos with high reliability

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2025-09-22 DOI:10.1016/j.snb.2025.138819

Hao Tan , Ying Wang , Jiacheng Song , Langhui Tao , Jinxiu Wei , Yuye Li , Xiaohong Liu , Chengxi Zhu

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Abstract

The rapid and reliable detection of pesticide residues play a pivotal role in safeguarding food safety. By integrating ratiometric electrochemical (EC) and electrochemical impedance spectroscopy (EIS) strategies, this work develops an all-in-one dual-mode aptasensor based on ferrocene-functionalized zeolitic imidazolate framework decorated with platinum nanoparticles (ZIF-8@Fc@PtNPs) for reliable detection of chlorpyrifos (CPS). ZIF-8@Fc@PtNPs were synthesized via a facile self-assembly approach, functioning as a dual-functional substrate to yield a reference signal (I_Fc) and amplify response signals. Complementary DNA (cDNA) was then immobilized via Pt-N bond, providing specific binding sites for assembling methylene blue-labelled CPS aptamer@Au nanoparticles (MB-CPS Apt@AuNPs). In the presence of CPS, specific bindings triggered the detachment of MB-CPS Apt@AuNPs, leading to measurable changes in the I_Fc/I_MB and electron transfer resistance (R_et). This dual-mode platform, by integrating ratiometric EC (based on I_Fc/I_MB) and EIS (based on R_et) strategies, allows for reciprocal validation of results derived from both modes, ensuring enhanced accuracy and reliability. The approach achieved high detection sensitivity for CPS within 30 pg mL⁻¹-30 ng mL⁻¹ (ratiometric EC mode) and 10 pg mL⁻¹-30 ng mL⁻¹ (EIS mode), coupled with good selectivity, reproducibility, and stability. Successful application in vegetable samples demonstrates its great potential for practical pesticide residue monitoring.

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二茂铁功能化ZIF-8@Pt纳米粒子工程双模适体传感器：高可靠性毒死蜱的比例电化学/阻抗检测

快速、可靠的农药残留检测对保障食品安全起着至关重要的作用。通过整合比例电化学（EC）和电化学阻抗谱（EIS）策略，本工作开发了一种基于二茂铁功能化的纳米铂修饰的沸石咪唑酸酯框架（ZIF-8@Fc@PtNPs）的一体化双模式感应传感器，用于可靠地检测毒死蜱（CPS）。ZIF-8@Fc@PtNPs通过简单的自组装方法合成，作为双功能底物产生参考信号（IFc）和放大响应信号。然后通过Pt-N键固定互补DNA (cDNA)，为组装亚甲基蓝标记的CPS aptamer@Au纳米颗粒（MB-CPS Apt@AuNPs）提供特定的结合位点。在CPS存在的情况下，特异性绑定触发MB-CPS Apt@AuNPs的脱离，导致IFc/IMB和电子转移阻力（Ret）的可测量变化。这种双模式平台通过整合比率EC（基于IFc/IMB）和EIS（基于Ret）策略，允许对两种模式得出的结果进行相互验证，确保提高准确性和可靠性。该方法在30 pg mL-1 ~ 30 ng mL-1（比率EC模式）和10 pg mL-1 ~ 30 ng mL-1 （EIS模式）范围内对CPS具有较高的检测灵敏度，具有良好的选择性、重复性和稳定性。在蔬菜样品中的成功应用表明了该方法在实际农药残留监测中的巨大潜力。

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.