Immunomodulatory effects of hexanoic extract of Narcissus tazetta flowers after subacute exposure to BALB/c mice through a phased approach immunotoxicity assessment

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2025-09-17 DOI:10.1016/j.tice.2025.103147

Seyed Mostafa Moshirian-Farahi , Fatemeh Forouzanfar , Hassan Rakhshandeh , Bahram Memar , Roghayeh Rashidi , Saeide Mansoori , Bamdad Riahi-Zanjani , Mahmood Sadeghi

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引用次数: 0

Abstract

Narcissus tazetta, a perennial herbaceous and medicinal plant, exhibits various pharmacological properties. The current study investigates the immunomodulatory effects of hexanoic extract of N. tazetta flowers (HENTF) in BALB/c mice immune system. Over a 14-day period, HENTF was administered orally at doses of 25 and 50 mg/kg. Evaluations of the immune system included histopathological examination of spleen and bone marrow, spleen cellularity, delayed type hypersensitivity (DTH) response, lymphocyte proliferation assay, hemagglutination titer (HA), and cytokines production. These findings suggest that HENTF exhibits immunostimulatory properties. The results demonstrated that higher dose of 50 mg/kg might enhance the immune responses on both cellular and humoral levels, with significant effects in the proliferation of lymphocytes. Mechanistic studies are necessary to precisely explore how HENTF exerts its immunostimulatory properties. Future investigations are recommended to evaluate the efficacy of HENTF as a co-adjuvant or immunostimulant in therapeutic applications for immune dysfunction.

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水仙花己酸提取物对BALB/c小鼠亚急性暴露后的免疫调节作用

水仙是一种多年生草本和药用植物，具有多种药理特性。本研究探讨了荆芥花己酸提取物（HENTF）对BALB/c小鼠免疫系统的调节作用。在14天的时间内，以25和50 mg/kg的剂量口服HENTF。免疫系统的评估包括脾和骨髓的组织病理学检查、脾细胞量、延迟型超敏反应（DTH）、淋巴细胞增殖试验、血凝滴度（HA）和细胞因子的产生。这些发现表明HENTF具有免疫刺激特性。结果表明，较高剂量50 mg/kg可增强细胞和体液水平的免疫应答，对淋巴细胞增殖有显著影响。机制研究是精确探索HENTF如何发挥其免疫刺激特性的必要条件。未来的研究建议评估HENTF作为辅助或免疫刺激剂治疗免疫功能障碍的疗效。

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.