Betaine promotes the osteogenic differentiation of SCAPs via inhibiting NUMB

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2025-09-15 DOI:10.1016/j.tice.2025.103142

Meiyue Wang , Jiaman Zhu , Xiaoyu Zheng , Zejie Wu , Runtao Gao

引用次数: 0

Abstract

Betaine, a known modulator of mesenchymal stem cells (MSCs) differentiation, has demonstrated potential in enhancing osteogenesis. However, its specific role in the osteogenic differentiation of stem cells from the apical papilla (SCAPs) has not been fully elucidated. In this study, we found that betaine (10 mM) significantly enhanced the osteogenesis of SCAPs, as evidenced by increased ALP activity and mineralized nodule formation (p < 0.05). Betaine downregulated NUMB expression, a negative regulator of osteogenic differentiation, during the induction process. Overexpression of NUMB counteracted the promotive effects of betaine on SCAPs osteogenesis and NUMB knockdown enhances betaine effects. These findings suggest that betaine regulates the osteogenic differentiation of SCAPs via inhibition of NUMB and may serve as a novel therapeutic approach for maxillofacial bone repair, with NUMB as a potential drug target.

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甜菜碱通过抑制NUMB促进SCAPs的成骨分化。

甜菜碱是一种已知的间充质干细胞（MSCs）分化调节剂，已被证明具有促进成骨的潜力。然而，其在根尖乳头（SCAPs）干细胞成骨分化中的具体作用尚未完全阐明。在这项研究中，我们发现甜菜碱（10 mM）显著促进SCAPs的成骨，这可以通过增加ALP活性和矿化结节形成来证明(p

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.