Sensing 5-HT by specific aptamer modified nanostructures in chronic restraint stress-induced depression mice

IF 4.6 2区医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL

Nanomedicine : nanotechnology, biology, and medicine Pub Date : 2025-09-18 DOI:10.1016/j.nano.2025.102857

Qian Chen , Jing Wei , Lanlan Ma , Jiameng Fang , Jiemin Zhao , Qian Meng

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Abstract

Depression is a severe psychiatric syndrome, characterized by its high prevalence and substantial socioeconomic impact. 5-Hydroxytryptamine (5-HT) has been correlated with both the pathogenesis and treatment of major depression. There is a lack of sensitive methods for detecting 5-HT levels in the brain to characterize depression onset and severity. Tetrahedral DNA nanostructures (TDNs) show promises as 5-HT sensors due to their ability to confer diverse target specificity and permeate the blood-brain barrier. In this study, we constructed a nanoscale complex, 5-HT-TDNs, by combining TDNs with a specific 5-HT aptamer and 6-Carboxyfluorescein. The 5-HT-TDNs targeted decreased 5-HT in the brains of mice subjected to chronic restraint stress-induced depression. Following fluoxetine administration to these mice, we observed a significant increase of 5-HT in brain tissue using 5-HT-TDNs. This work illustrates the potential for sensitively and stably indicating neurotransmitters such as 5-HT in individuals with depression.

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特异性适配体修饰纳米结构对慢性抑制性应激性抑郁症小鼠5-羟色胺的感知。

抑郁症是一种严重的精神综合征，其特点是发病率高，社会经济影响大。5-羟色胺（5-HT）与重度抑郁症的发病机制和治疗相关。缺乏检测大脑中5-羟色胺水平的灵敏方法来表征抑郁症的发病和严重程度。由于四面体DNA纳米结构（tdn）具有不同的靶标特异性和穿透血脑屏障的能力，因此有望成为5-羟色胺传感器。在这项研究中，我们通过将tdn与特定的5-HT适配体和6-羧基荧光素结合，构建了一个纳米级复合物，5-HT- tdn。5-HT- tdn靶向降低慢性抑制性应激性抑郁症小鼠大脑中的5-HT。在给这些小鼠氟西汀后，我们观察到脑组织中5-HT的显著增加。这项工作说明了在抑郁症患者中敏感而稳定地指示神经递质（如5-HT）的潜力。

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来源期刊

Nanomedicine : nanotechnology, biology, and medicine 工程技术-纳米科技

CiteScore

11.10

自引率

0.00%

发文量

133

审稿时长

42 days

期刊介绍： The mission of Nanomedicine: Nanotechnology, Biology, and Medicine (Nanomedicine: NBM) is to promote the emerging interdisciplinary field of nanomedicine. Nanomedicine: NBM is an international, peer-reviewed journal presenting novel, significant, and interdisciplinary theoretical and experimental results related to nanoscience and nanotechnology in the life and health sciences. Content includes basic, translational, and clinical research addressing diagnosis, treatment, monitoring, prediction, and prevention of diseases.