Three-dimensional genome reorganization in hematopoietic stem cells.

IF 2.1 4区医学 Q2 HEMATOLOGY

Experimental hematology Pub Date : 2025-09-18 DOI:10.1016/j.exphem.2025.105249

Akihiro Nakajima, Keisuke Kirito, Mio Nakanishi, Naoya Takayama

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引用次数: 0

Abstract

Hematopoietic stem cells (HSCs) possess unique characteristics that distinguish them from other hematopoietic progenitor cells, including self-renewal capacity, multipotency, stress response, metabolism, and deep quiescence. Recent advances have significantly enhanced our understanding of the epigenomic states that define these properties. HSCs undergo profound changes in their three-dimensional (3D) genome reorganization throughout development, differentiation, and responses to stimuli. Recent advancements in chromatin conformation capture techniques that require only a small number of cells have provided detailed insights into these dynamic processes. This review explored the latest discoveries in the 3D genome reorganization in HSCs, with a focus on chromatin remodeling during key transitions, including fetal-to-adult development, quiescence-to-activation, differentiation, and aging. We discussed the roles of key transcription factors, epigenetic modifiers, and structural proteins in shaping the 3D genome landscape. Additionally, we examined how alterations in the 3D genome organization impact HSC function and dysfunction in hematologic disorders. Finally, we highlighted future directions in this rapidly evolving field, emphasizing the potential implications of 3D genome research for targeted therapies in hematology.

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造血干细胞的三维基因组重组。

造血干细胞（hsc）具有不同于其他造血祖细胞的独特特性，包括自我更新能力、多能性、应激反应、代谢和深度静止。最近的进展大大提高了我们对定义这些特性的表观基因组状态的理解。造血干细胞在发育、分化和对刺激的反应过程中，其三维（3D）基因组重组经历了深刻的变化。染色质构象捕获技术的最新进展只需要少量的细胞，为这些动态过程提供了详细的见解。本文综述了造血干细胞中三维基因组重组的最新发现，重点关注关键转变过程中的染色质重塑，包括胎儿到成人发育、静止到激活、分化和衰老。我们讨论了关键转录因子、表观遗传修饰因子和结构蛋白在塑造三维基因组景观中的作用。此外，我们研究了3D基因组组织的改变如何影响血液学疾病中的HSC功能和功能障碍。最后，我们强调了这一快速发展领域的未来方向，强调了3D基因组研究对血液学靶向治疗的潜在意义。摘要：造血干细胞（Hematopoietic stem cells, hsc）具有自我更新、多能性、应激反应、代谢和静止等特性。染色质构象捕获的进展揭示了它们的三维基因组组织在发育、激活、分化和衰老过程中是如何变化的。本文综述了形成HSC基因组结构的染色质重塑、关键转录因子、表观遗传修饰因子和结构蛋白，以及其破坏如何导致血液系统疾病，并强调了治疗前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Experimental hematology 医学-血液学

CiteScore

5.30

自引率

0.00%

发文量

审稿时长

58 days

期刊介绍： Experimental Hematology publishes new findings, methodologies, reviews and perspectives in all areas of hematology and immune cell formation on a monthly basis that may include Special Issues on particular topics of current interest. The overall goal is to report new insights into how normal blood cells are produced, how their production is normally regulated, mechanisms that contribute to hematological diseases and new approaches to their treatment. Specific topics may include relevant developmental and aging processes, stem cell biology, analyses of intrinsic and extrinsic regulatory mechanisms, in vitro behavior of primary cells, clonal tracking, molecular and omics analyses, metabolism, epigenetics, bioengineering approaches, studies in model organisms, novel clinical observations, transplantation biology and new therapeutic avenues.