Structural Characterization and Anti-inflammatory Activity of a Polysaccharide Produced by Endophytic Fungus Talaromyces Sp. CCTCC M 2025051

IF 5 3区工程技术 Q2 ENGINEERING, ENVIRONMENTAL

Journal of Polymers and the Environment Pub Date : 2025-09-08 DOI:10.1007/s10924-025-03664-8

Zichao Wang, Yixin Shi, Xin Liu, Na Li, Jiaqi Wang, Hongtao Zhang, Yingjie Feng, Yongming Xu, Qinggong Chen, Changxing Liu, Tao Liu, Dandan Liu, Qi Wang, Jinchu Yang, Lemei An

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引用次数: 0

Abstract

Endophytic fungi and their secondary metabolites represent a valuable microbial resource with diverse bioactivities and potential applications. In this study, Talaromyces sp. CCTCC M2025051 was isolated from wheat bran, and its extracellular polysaccharide (TSP) was investigated. Compositional analysis revealed that the yield of TSP was 3.34 ± 0.17 g/L, comprising 93.11 ± 2.93% carbohydrate and 2.82 ± 0.07% protein. TSP was identified as a heteropolysaccharide composed of arabinose, galactose, glucose, mannose, and glucuronic acid in a molar ratio of 0.0985: 4.0208: 35.6949: 2.0528: 0.1718, with a molecular weight of 235.187 kDa and a polydispersity of 1.886. In vitro assays demonstrated that TSP effectively scavenged ABTS, DPPH, hydroxyl, and superoxide radicals in a concentration-dependent manner. Furthermore, TSP exhibited significant anti-inflammatory activity in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages by reducing the production of nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β). This effect was accompanied by the modulation of oxidative stress markers, including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA). This work provides a scientific basis for the potential application of TSP as an antioxidant and anti-inflammatory agent.

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内生真菌Talaromyces Sp. CCTCC M 2025051多糖的结构表征及抗炎活性

内生真菌及其次生代谢产物是一种具有丰富生物活性和潜在应用价值的微生物资源。本研究从小麦麸皮中分离得到Talaromyces sp. CCTCC M2025051，并对其胞外多糖（TSP）进行了研究。结果表明，产率为3.34±0.17 g/L，碳水化合物含量为93.11±2.93%，蛋白质含量为2.82±0.07%。经鉴定，TSP是一种由阿拉伯糖、半乳糖、葡萄糖、甘露糖和葡萄糖醛酸组成的杂多糖，摩尔比为0.0985:4.0208:35.6949:2.0528:0.1718，分子量为235.187 kDa，多元度为1.886。体外实验表明，TSP以浓度依赖性的方式有效清除ABTS、DPPH、羟基和超氧自由基。此外，TSP通过减少一氧化氮（NO）、肿瘤坏死因子-α (TNF-α)、白细胞介素-6 （IL-6）和白细胞介素-1β (IL-1β)的产生，对脂多糖（LPS）诱导的RAW 264.7巨噬细胞具有显著的抗炎活性。这种效应伴随着氧化应激标志物的调节，包括超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）和丙二醛（MDA）。本研究为TSP作为抗氧化剂和抗炎剂的潜在应用提供了科学依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Polymers and the Environment 工程技术-高分子科学

CiteScore

9.50

自引率

7.50%

发文量

297

审稿时长

9 months

期刊介绍： The Journal of Polymers and the Environment fills the need for an international forum in this diverse and rapidly expanding field. The journal serves a crucial role for the publication of information from a wide range of disciplines and is a central outlet for the publication of high-quality peer-reviewed original papers, review articles and short communications. The journal is intentionally interdisciplinary in regard to contributions and covers the following subjects - polymers, environmentally degradable polymers, and degradation pathways: biological, photochemical, oxidative and hydrolytic; new environmental materials: derived by chemical and biosynthetic routes; environmental blends and composites; developments in processing and reactive processing of environmental polymers; characterization of environmental materials: mechanical, physical, thermal, rheological, morphological, and others; recyclable polymers and plastics recycling environmental testing: in-laboratory simulations, outdoor exposures, and standardization of methodologies; environmental fate: end products and intermediates of biodegradation; microbiology and enzymology of polymer biodegradation; solid-waste management and public legislation specific to environmental polymers; and other related topics.